DIDO1 Antibody - #DF3612

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Product: DIDO1 Antibody
Catalog: DF3612
Description: Rabbit polyclonal antibody to DIDO1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse, Rabbit
Mol.Wt.: 244 KD; 244kD(Calculated).
Uniprot: Q9BTC0
RRID: AB_2835984

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
DIDO1 Antibody detects endogenous levels of total DIDO1.
RRID:
AB_2835984
Cite Format: Affinity Biosciences Cat# DF3612, RRID:AB_2835984.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BYE 1; BYE1; C20orf158; DATF 1; DATF-1; Death associated transcription factor 1; Death inducer obliterator 1; Death-associated transcription factor 1; Death-inducer obliterator 1; DIDO 1; DIDO 2; DIDO 3; DIDO1; DIDO1_HUMAN; DIDO2; DIDO3; DIO 1; DIO-1; DIO1; dJ885L7.8; DKFZp434P1115; FLJ11265; hDido 1; hDido1; KIAA0333; MGC16140;

Immunogens

Immunogen:
Uniprot:

Q9BTC0(DIDO1_HUMAN) >>Visit HPA database.

Gene(ID):
DIDO1(11083)
Expression:
Q9BTC0 DIDO1_HUMAN:

Ubiquitous.

Sequence:
MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEPPPPQQQLGLSLRRSGRQPKRTERVEQFLTIARRRGRRSMPVSLEDSGEPTSCPATDAETASEGSVESASETRSGPQSASTAVKERPASSEKVKGGDDHDDTSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAETVGSEASDTVEGVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEEPGDLGRPKPECEGYDPNALYCICRQPHNNRFMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDCTSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKPSLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACESSTPSWASDHNYNAVKPEKTAAPSPSLLYKSTKEDRRSEEKAAAMAASKKTAPPGSAVGKQPAPRNLVPKKSSFANVAAATPAIKKPPSGFKGTIPKRPWLSATPSSGASAARQAGPAPAAATAASKKFPGSAALVGAVRKPVVPSVPMASPAPGRLGAMSAAPSQPNSQIRQNIRRSLKEILWKRVNDSDDLIMTENEVGKIALHIEKEMFNLFQVTDNRYKSKYRSIMFNLKDPKNQGLFHRVLREEISLAKLVRLKPEELVSKELSTWKERPARSVMESRTKLHNESKKTAPRQEAIPDLEDSPPVSDSEEQQESARAVPEKSTAPLLDVFSSMLKDTTSQHRAHLFDLNCKICTGQVPSAEDEPAPKKQKLSASVKKEDLKSKHDSSAPDPAPDSADEVMPEAVPEVASEPGLESASHPNVDRTYFPGPPGDGHPEPSPLEDLSPCPASCGSGVVTTVTVSGRDPRTAPSSSCTAVASAASRPDSTHMVEARQDVPKPVLTSVMVPKSILAKPSSSPDPRYLSVPPSPNISTSESRSPPEGDTTLFLSRLSTIWKGFINMQSVAKFVTKAYPVSGCFDYLSEDLPDTIHIGGRIAPKTVWDYVGKLKSSVSKELCLIRFHPATEEEEVAYISLYSYFSSRGRFGVVANNNRHVKDLYLIPLSAQDPVPSKLLPFEGPGLESPRPNIILGLVICQKIKRPANSGELDKMDEKRTRLQPEEADVPAYPKVATVPQSEKKPSKYPLCSADAAVSTTPPGSPPPPPPLPEPPVLKVLSSLKPAAPSPATAATTAAAASTAASSTASSASKTASPLEHILQTLFGKKKSFDPSAREPPGSTAGLPQEPKTTAEDGVPAPPLLDPIVQQFGQFSKDKALEEEEDDRPYDPEEEYDPERAFDTQLVERGRRHEVERAPEAAAAEREEVAYDPEDETILEEAKVTVDDLPNRMCADVRRNSVERPAEPVAGAATPSLVEQQKMLEELNKQIEEQKRQLEEQEEALRQQRAAVGVSMAHFSVSDALMSPPPKSSLPKAELFQQEQQSADKPASLPPASQASNHRDPRQARRLATETGEGEGEPLSRLSARGAQGALPERDASRGGLVGQAPMPVPEEKEPASSPWASGEKPPAGSEQDGWKAEPGEGTRPATVGDSSARPARRVLLPTPPCGALQPGFPLQHDGERDPFTCPGFASQDKALGSAQYEDPRNLHSAGRSSSPAGETEGDREPQARPGEGTAPLPPPGQKVGGSQPPFQGQREPGPHALGMSGLHGPNFPGPRGPAPPFPEENIASNDGPRGPPPARFGAQKGPIPSLFSGQHGPPPYGDSRGPSPSYLGGPRGVAPSQFEERKDPHGEKREFQDAPYNEVTGAPAQFEGTEQAPFLGSRGGAPFQFGGQRRPLLSQLKGPRGGPPPSQFGGQRGPPPGHFVGPRGPHPSQFETARGPHPNQFEGPRGQAPNFMPGPRGIQPQQFEDQRVHSPPRFTNQRAPAPLQFGGLRGSAPFSEKNEQTPSRFHFQGQAPQVMKPGPRPLLELPSHPPQHRKDRWEEAGPPSALSSSAPGQGPEADGQWASADFREGKGHEYRNQTFEGRQRERFDVGPKEKPLEEPDAQGRASEDRRRERERGRNWSRERDWDRPREWDRHRDKDSSRDWDRNRERSANRDREREADRGKEWDRSRERSRNRERERDRRRDRDRSRSRERDRDKARDRERGRDRKDRSKSKESARDPKPEASRASDAGTASQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BTC0 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S8 Phosphorylation
K21 Acetylation
T32 Phosphorylation
T33 Phosphorylation
S60 Phosphorylation
S88 Phosphorylation
S92 Phosphorylation
S101 Phosphorylation
C102 S-Nitrosylation
T109 Phosphorylation
S111 Phosphorylation
S114 Phosphorylation
S117 Phosphorylation
S123 Phosphorylation
T151 Phosphorylation
S152 Phosphorylation
S154 Phosphorylation
S156 Phosphorylation
S203 Phosphorylation
K247 Sumoylation
C350 S-Nitrosylation
K359 Ubiquitination
S377 Phosphorylation
K383 Ubiquitination
K427 Acetylation
K427 Ubiquitination
K433 Ubiquitination
S464 Phosphorylation
S465 Phosphorylation
K474 Acetylation
S501 Phosphorylation
T502 Phosphorylation
S504 Phosphorylation
S507 Phosphorylation
K515 Ubiquitination
T519 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
S555 Phosphorylation
S572 Phosphorylation
T603 Phosphorylation
S609 Phosphorylation
K626 Acetylation
K627 Acetylation
S645 Phosphorylation
S650 Phosphorylation
S660 Phosphorylation
S677 Phosphorylation
K679 Ubiquitination
Y721 Phosphorylation
S723 Phosphorylation
K736 Ubiquitination
K753 Ubiquitination
S781 Phosphorylation
S805 Phosphorylation
S809 Phosphorylation
S811 Phosphorylation
S817 Phosphorylation
S862 Phosphorylation
S898 Phosphorylation
S918 Phosphorylation
S920 Phosphorylation
S947 Phosphorylation
S1011 Phosphorylation
K1015 Acetylation
K1015 Ubiquitination
S1017 Phosphorylation
S1018 Phosphorylation
S1019 Phosphorylation
S1030 Phosphorylation
S1034 Phosphorylation
T1035 Phosphorylation
S1036 Phosphorylation
R1039 Methylation
S1040 Phosphorylation
K1072 Ubiquitination
K1100 Ubiquitination
K1108 Ubiquitination
S1205 Phosphorylation
Y1228 Phosphorylation
S1237 Phosphorylation
S1242 Phosphorylation
Y1244 Phosphorylation
S1248 Phosphorylation
S1254 Phosphorylation
T1255 Phosphorylation
T1256 Phosphorylation
S1260 Phosphorylation
S1285 Phosphorylation
T1310 Phosphorylation
S1312 Phosphorylation
S1327 Phosphorylation
S1331 Phosphorylation
S1338 Phosphorylation
S1456 Phosphorylation
T1469 Phosphorylation
S1471 Phosphorylation
S1522 Phosphorylation
S1541 Phosphorylation
R1597 Methylation
S1616 Phosphorylation
S1617 Phosphorylation
T1662 Phosphorylation
S1697 Phosphorylation
Y1700 Phosphorylation
S1712 Phosphorylation
S1713 Phosphorylation
S1714 Phosphorylation
T1719 Phosphorylation
R1775 Methylation
R1793 Methylation
R1799 Methylation
K1804 Methylation
S1823 Phosphorylation
R1824 Methylation
S1827 Phosphorylation
R1835 Methylation
R1845 Methylation
K1846 Methylation
K1852 Acetylation
R1882 Methylation
R1893 Methylation
R1894 Methylation
K1901 Acetylation
R1904 Methylation
R1927 Methylation
R1938 Methylation
R1949 Methylation
R1960 Methylation
R1971 Methylation
S1974 Phosphorylation
R1977 Methylation
R1982 Methylation
R1993 Methylation
R2008 Methylation
K2020 Methylation
R2024 Methylation
S2110 Phosphorylation
S2229 Phosphorylation
S2232 Phosphorylation

Research Backgrounds

Function:

Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development.

Displaces isoform 4 at the onset of differentiation, required for repression of stemness genes.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Note: Translocates to the nucleus after pro-apoptotic stimuli (By similarity). Translocates to the mitotic spindle upon loss of interaction with H3K4me3 during early mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts specifically (via PHD-type zinc finger) with histone H3 that is trimethylated at 'Lys-4' (H3K4me3), histone phosphorylation at 'Thr-3' or 'Thr-6' disrupts this binding and promotes translocation of DIDO1 from chromatin to the mitotic spindle during mitosis.

Family&Domains:

The PHD-type zinc finger forms an aromatic cage around H3K4me3.

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