BARD1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells, using BARD1 antibody.

Product:	BARD1 Antibody
Catalog:	DF3605
Description:	Rabbit polyclonal antibody to BARD1
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	79 KD; 87kD(Calculated).
Uniprot:	Q99728
RRID:	AB_2835977

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

BARD1 Antibody detects endogenous levels of total BARD1.

RRID:

AB_2835977
Cite Format: Affinity Biosciences Cat# DF3605, RRID:AB_2835977.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BARD-1; Bard1; BARD1_HUMAN; BRCA1 associated RING domain 1; BRCA1 associated RING domain gene 1; BRCA1 associated RING domain protein 1; BRCA1-associated RING domain protein 1;

Immunogens

Immunogen:

Uniprot:

Q99728(BARD1_HUMAN) >>Visit HPA database.

Gene(ID):

BARD1(580)

Sequence:

Q99728 BARD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPDNRQPRNRQPRIRSGNEPRSAPAMEPDGRGAWAHSRAALDRLEKLLRCSRCTNILREPVCLGGCEHIFCSNCVSDCIGTGCPVCYTPAWIQDLKINRQLDSMIQLCSKLRNLLHDNELSDLKEDKPRKSLFNDAGNKKNSIKMWFSPRSKKVRYVVSKASVQTQPAIKKDASAQQDSYEFVSPSPPADVSERAKKASARSGKKQKKKTLAEINQKWNLEAEKEDGEFDSKEESKQKLVSFCSQPSVISSPQINGEIDLLASGSLTESECFGSLTEVSLPLAEQIESPDTKSRNEVVTPEKVCKNYLTSKKSLPLENNGKRGHHNRLSSPISKRCRTSILSTSGDFVKQTVPSENIPLPECSSPPSCKRKVGGTSGRKNSNMSDEFISLSPGTPPSTLSSSSYRRVMSSPSAMKLLPNMAVKRNHRGETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGKVWKAPSSWFIDCVMSFELLPLDS

PTMs - Q99728 As Substrate

Q99728

Site	PTM Type	Enzyme	Source
K46	Sumoylation		Uniprot
K110	Ubiquitination		Uniprot
K124	Acetylation		Uniprot
K124	Ubiquitination		Uniprot
K130	Ubiquitination		Uniprot
K139	Ubiquitination		Uniprot
K144	Sumoylation		Uniprot
S148	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
K152	Acetylation		Uniprot
K160	Ubiquitination		Uniprot
K170	Sumoylation		Uniprot
K170	Ubiquitination		Uniprot
S184	Phosphorylation		Uniprot
S186	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
K217	Sumoylation		Uniprot
K217	Ubiquitination		Uniprot
K224	Ubiquitination		Uniprot
S244	Phosphorylation		Uniprot
S251	Phosphorylation	P06493 (CDK1) , P24941 (CDK2)	Uniprot
S288	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
T299	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
K305	Sumoylation		Uniprot
Y307	Phosphorylation		Uniprot
S313	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S342	Phosphorylation		Uniprot
S344	Phosphorylation		Uniprot
S364	Phosphorylation		Uniprot
S367	Phosphorylation		Uniprot
K371	Acetylation		Uniprot
S391	Phosphorylation		Uniprot
T394	Phosphorylation		Uniprot
T398	Phosphorylation		Uniprot
S402	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
S412	Phosphorylation		Uniprot
K423	Sumoylation		Uniprot
K423	Ubiquitination		Uniprot
T430	Phosphorylation		Uniprot
S443	Phosphorylation		Uniprot
Y446	Phosphorylation		Uniprot
K457	Ubiquitination		Uniprot
K484	Ubiquitination		Uniprot
S496	Phosphorylation		Uniprot
K548	Sumoylation		Uniprot
K548	Ubiquitination		Uniprot
S586	Phosphorylation		Uniprot
K596	Ubiquitination		Uniprot
K650	Ubiquitination		Uniprot
K688	Ubiquitination		Uniprot
S704	Phosphorylation		Uniprot
T714	Phosphorylation		Uniprot
T719	Phosphorylation		Uniprot
T734	Phosphorylation		Uniprot

Research Backgrounds

Q99728_BARD1_HUMAN

Function:

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.

PTMs:

Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.

Subcellular Location:

Nucleus.
Note: During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.

Subunit Structure:

Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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BARD1 Antibody - #DF3605