IL17RA Antibody - #DF3602

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Product: IL17RA Antibody
Catalog: DF3602
Description: Rabbit polyclonal antibody to IL17RA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 90 KD; 96kD(Calculated).
Uniprot: Q96F46
RRID: AB_2835974

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(82%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IL17RA Antibody detects endogenous levels of total IL17RA.
RRID:
AB_2835974
Cite Format: Affinity Biosciences Cat# DF3602, RRID:AB_2835974.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CANDF5; CD217; CD217 antigen; CDw217; CTLA8; HIL 17R; hIL17R; I17RA_HUMAN; IL 17 receptor A; IL 17 receptor; IL 17RA; IL-17 receptor A; IL-17RA; IL17; IL17A receptor; IL17R; IL17RA; IMD51; Interleukin 17 receptor A; Interleukin-17 receptor A; MGC10262;

Immunogens

Immunogen:
Uniprot:

Q96F46(I17RA_HUMAN) >>Visit HPA database.

Gene(ID):
IL17RA(23765)
Expression:
Q96F46 I17RA_HUMAN:

Widely expressed.

Sequence:
MGAARSPPSAVPGPLLGLLLLLLGVLAPGGASLRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLWVYWFITGISILLVGSVILLIVCMTWRLAGPGSEKYSDDTKYTDGLPAADLIPPPLKPRKVWIIYSADHPLYVDVVLKFAQFLLTACGTEVALDLLEEQAISEAGVMTWVGRQKQEMVESNSKIIVLCSRGTRAKWQALLGRGAPVRLRCDHGKPVGDLFTAAMNMILPDFKRPACFGTYVVCYFSEVSCDGDVPDLFGAAPRYPLMDRFEEVYFRIQDLEMFQPGRMHRVGELSGDNYLRSPGGRQLRAALDRFRDWQVRCPDWFECENLYSADDQDAPSLDEEVFEEPLLPPGTGIVKRAPLVREPGSQACLAIDPLVGEEGGAAVAKLEPHLQPRGQPAPQPLHTLVLAAEEGALVAAVEPGPLADGAAVRLALAGEGEACPLLGSPGAGRNSVLFLPVDPEDSPLGSSTPMASPDLLPEDVREHLEGLMLSLFEQSLSCQAQGGCSRPAMVLTDPHTPYEEEQRQSVQSDQGYISRSSPQPPEGLTEMEEEEEEEQDPGKPALPLSPEDLESLRSLQRQLLFRQLQKNSGWDTMGSESEGPSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
82
Chicken
50
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96F46 As Substrate

Site PTM Type Enzyme
N49 N-Glycosylation
N54 N-Glycosylation
N67 N-Glycosylation
N225 N-Glycosylation
S264 Phosphorylation
N265 N-Glycosylation
K360 Ubiquitination
S554 Phosphorylation
S629 Phosphorylation
S708 Phosphorylation
S726 Phosphorylation
T780 Phosphorylation P49841 (GSK3B)
S792 Phosphorylation
Y796 Phosphorylation
S798 Phosphorylation
S801 Phosphorylation

Research Backgrounds

Function:

Receptor for IL17A. Receptor for IL17F. Binds to IL17A with higher affinity than to IL17F. Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC. Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC. Receptor for IL17C as part of a heterodimeric complex with IL17RE. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6.

PTMs:

Glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Forms heterodimers with IL17RE; the heterodimer binds IL17C. Forms heterodimers with IL17RC; the heterodimer binds IL17A and IL17F homodimers as well as the heterodimer formed by IL17A and IL17F.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). Development and Validation of a 7-Gene Inflammatory Signature Forecasts Prognosis and Diverse Immune Landscape in Lung Adenocarcinoma. Frontiers in Molecular Biosciences (PubMed: 35372503) [IF=5.0]

Application: WB    Species: human    Sample: bronchial epithelial cell line (16-HBS) and lung adenocarcinoma cell line (A549）

FIGURE 10 | Protein expression of seven model genes. (A–C) Immunohistochemical staining images of MMP14 (A), PCDH7 (B), and LAMP3 (C) from the HPA database. (D) Western blotting results shows the protein expression of BTG2, TLR2, CCL20, and IL7R in a normal bronchial epithelial cell line (16-HBS) and lung adenocarcinoma cell line (A549).
2). IL-17A exacerbates cisplatin-based resistance of OVCA via upregulating the expression of ABCG2 and MDR1 through Gli1-mediated Hh signaling. Oncotarget

Application: WB    Species: Human    Sample: A2780

Figure 1: rhIL-17A has no effect on OVCA cell proliferation. A. Protein levels of IL-17RA in five human OVCA cell lines, including A2780, OVCAR3, SKOV3, HO8910, HO8910/PM, and a mouse OVCA cell line ID8 analyzed by western blotting. Protein lysates from OVCA cells were prepared and analyzed by western blotting. Representative result from three independent experiments was showed. B. rhIL-17A had no effect on the proliferation of A2780 and OVCAR3 cells at given concentration (0-100ng/ml) at designated time points (12, 24, 48, 72h) by MTT assay. Data represent means±SD from three independent experiments.

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