HSPB2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from rat brain, using HSPB2 Antibody.

Product:	HSPB2 Antibody
Catalog:	DF3601
Description:	Rabbit polyclonal antibody to HSPB2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog
Mol.Wt.:	24 KD; 20kD(Calculated).
Uniprot:	Q16082
RRID:	AB_2835973

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

HSPB2 Antibody detects endogenous levels of total HSPB2.

RRID:

AB_2835973
Cite Format: Affinity Biosciences Cat# DF3601, RRID:AB_2835973.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DMPK-binding protein; Heat shock 27kD protein 2; Heat shock 27kDa protein 2; Heat shock protein beta-2; Heat-shock protein beta-2; Hs.78846; HSP27; HspB2; HSPB2_HUMAN; LOH11CR1K; MGC133245; MKBP;

Immunogens

Immunogen:

Uniprot:

Q16082(HSPB2_HUMAN) >>Visit HPA database.

Gene(ID):

HSPB2(3316)

Expression:

Q16082 HSPB2_HUMAN:

Expressed preferentially in skeletal muscle and heart but not in the lens.

Sequence:

Q16082 HSPB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGRSVPHAHPATAEYEFANPSRLGEQRFGEGLLPEEILTPTLYHGYYVRPRAAPAGEGSRAGASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLPADVDPWRVRAALSHDGILNLEAPRGGRHLDTEVNEVYISLLPAPPDPEEEEEAAIVEP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16082 As Substrate

Q16082

Site	PTM Type	Source
S5	Phosphorylation	Uniprot
Y16	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
Y44	Phosphorylation	Uniprot
R52	Methylation	Uniprot
S60	Phosphorylation	Uniprot
R61	Methylation	Uniprot
S65	Phosphorylation	Uniprot

Research Backgrounds

Q16082_HSPB2_HUMAN

Function:

May regulate the kinase DMPK.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Localizes to nuclear foci.

Tissue Specificity:

Expressed preferentially in skeletal muscle and heart but not in the lens.

Subunit Structure:

Interacts with DMPK; may enhance its kinase activity.

Family&Domains:

Belongs to the small heat shock protein (HSP20) family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HSPB2 Antibody - #DF3601