GBP1 Antibody - #DF3598

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Product: GBP1 Antibody
Catalog: DF3598
Description: Rabbit polyclonal antibody to GBP1
Application: WB IF/ICC
Reactivity: Human
Prediction: Horse, Rabbit
Mol.Wt.: 68 KD; 68kD(Calculated).
Uniprot: P32455
RRID: AB_2835970

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(83%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
GBP1 Antibody detects endogenous levels of total GBP1.
RRID:
AB_2835970
Cite Format: Affinity Biosciences Cat# DF3598, RRID:AB_2835970.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GBP 1; GBP-1; GBP1; GBP1_HUMAN; GTP binding protein 1; GTP-binding protein 1; Guanine nucleotide binding protein 1; Guanine nucleotide-binding protein 1; Guanylate binding protein 1; Guanylate binding protein 1 interferon inducible 67kDa; Guanylate binding protein 1 interferon inducible; HuGBP 1; HuGBP-1; HuGBP1; Interferon induced guanylate binding protein 1; Interferon-induced guanylate-binding protein 1; OTTHUMP00000012352;

Immunogens

Immunogen:
Uniprot:

P32455(GBP1_HUMAN) >>Visit HPA database.

Gene(ID):
GBP1(2633)
Sequence:
MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Horse
83
Pig
75
Dog
57
Chicken
50
Zebrafish
36
Bovine
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32455 As Substrate

Site PTM Type Enzyme
S52 Phosphorylation
S66 Phosphorylation
T70 Phosphorylation
T75 Phosphorylation
S154 Phosphorylation
S156 Phosphorylation
S157 Phosphorylation
T218 Phosphorylation
K278 Ubiquitination
Y335 Phosphorylation
S371 Phosphorylation
S372 Phosphorylation
K374 Ubiquitination
K382 Ubiquitination
Y427 Phosphorylation
K453 Ubiquitination
Y464 Phosphorylation
S523 Phosphorylation
K544 Methylation
K544 Ubiquitination
K553 Acetylation
K562 Acetylation
K562 Ubiquitination
K573 Ubiquitination
K582 Ubiquitination

Research Backgrounds

Function:

Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.

PTMs:

Isoprenylation is required for proper subcellular location.

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Cell membrane. Secreted.
Note: Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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