SNT2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using SNT2 Antibody.

Product:	SNT2 Antibody
Catalog:	DF3597
Description:	Rabbit polyclonal antibody to SNT2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	54 KD; 54kD(Calculated).
Uniprot:	O43559
RRID:	AB_2835969

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(100%), Horse(80%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

SNT2 Antibody detects endogenous levels of total SNT2.

RRID:

AB_2835969
Cite Format: Affinity Biosciences Cat# DF3597, RRID:AB_2835969.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FGFR signalling adaptor SNT2; FGFR substrate 3; Fibroblast growth factor receptor substrate 3; FRS2 beta; FRS2B; FRS3; FRS3 protein; MGC17167; SNT 2; Suc1 associated neurotrophic factor target 2 (FGFR signalling adaptor); Suc1 associated neurotrophic factor target 2;

Immunogens

Immunogen:

Uniprot:

O43559(FRS3_HUMAN) >>Visit HPA database.

Gene(ID):

FRS3(10817)

Sequence:

O43559 FRS3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSCCSCLNRDSVPDNHPTKFKVTNVDDEGVELGSGVMELTQSELVLHLHRREAVRWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCSRAEEIFNLLQDLMQCNSINVMEEPVIITRNSHPAELDLPRAPQPPNALGYTVSSFSNGCPGEGPRFSAPRRLSTSSLRHPSLGEESTHALIAPDEQSHTYVNTPASEDDHRRGRHCLQPLPEGQAPFLPQARGPDQRDPQVFLQPGQVKFVLGPTPARRHMVKCQGLCPSLHDPPHHNNNNEAPSECPAQPKCTYENVTGGLWRGAGWRLSPEEPGWNGLAHRRAALLHYENLPPLPPVWESQAQQLGGEAGDDGDSRDGLTPSSNGFPDGEEDETPLQKPTSTRAAIRSHGSFPVPLTRRRGSPRVFNFDFRRPGPEPPRQLNYIQVELKGWGGDRPKGPQNPSSPQAPMPTTHPARSSDSYAVIDLKKTVAMSNLQRALPRDDGTARKTRHNSTDLPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Horse

Pig

Rabbit

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43559 As Substrate

O43559

Site	PTM Type	Enzyme	Source
G2	Myristoylation		Uniprot
S73	Phosphorylation		Uniprot
S76	Phosphorylation		Uniprot
T166	Phosphorylation		Uniprot
S167	Phosphorylation		Uniprot
Y192	Phosphorylation		Uniprot
Y287	Phosphorylation		Uniprot
Y322	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
Y417	Phosphorylation	Q16620 (NTRK2)	Uniprot
Y455	Phosphorylation	Q16620 (NTRK2)	Uniprot

Research Backgrounds

O43559_FRS3_HUMAN

Function:

Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.

PTMs:

Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by BFGF or NGFB.

Subcellular Location:

Membrane>Lipid-anchor.

Subunit Structure:

Binds NTRK1 (By similarity). Binds FGFR1, NGFR, GRB2, PTPN11 and ERK2.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SNT2 Antibody - #DF3597