Cytochrome P450 51A1 Antibody - #DF3591

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Product: Cytochrome P450 51A1 Antibody
Catalog: DF3591
Description: Rabbit polyclonal antibody to Cytochrome P450 51A1
Application: WB
Reactivity: Human
Prediction: Pig, Dog
Mol.Wt.: 57 KD; 57kD(Calculated).
Uniprot: Q16850
RRID: AB_2835963

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 51A1 Antibody detects endogenous levels of total Cytochrome P450 51A1.
RRID:
AB_2835963
Cite Format: Affinity Biosciences Cat# DF3591, RRID:AB_2835963.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CP51; CP51A_HUMAN; CYP51; CYP51A1; CYPL1; CYPLI; Cytochrome P450 51; Cytochrome P450 51A1; Cytochrome P450 family 51 subfamily A member 1; Cytochrome P450 family 51 subfamily A polypeptide 1; Cytochrome P450, 51 (lanosterol 14 alpha demethylase); Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; LDM; P450 14DM; P450L1; Sterol 14-alpha demethylase;

Immunogens

Immunogen:
Uniprot:

Q16850(CP51A_HUMAN) >>Visit HPA database.

Gene(ID):
CYP51A1(1595)
Expression:
Q16850 CP51A_HUMAN:

Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Sequence:
MLLLGLLQAGGSVLGQAMEKVTGGNLLSMLLIACAFTLSLVYLIRLAAGHLVQLPAGVKSPPYIFSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFESWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQKKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Dog
88
Horse
75
Bovine
75
Sheep
75
Rabbit
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16850 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
K59 Sumoylation
S60 Phosphorylation
S66 Phosphorylation
K79 Sumoylation
K79 Ubiquitination
K91 Ubiquitination
K121 Ubiquitination
Y131 Phosphorylation
S132 Phosphorylation
T136 Phosphorylation
K141 Ubiquitination
Y145 Phosphorylation
K156 Ubiquitination
K157 Ubiquitination
K160 Ubiquitination
K169 Ubiquitination
K177 Ubiquitination
K180 Ubiquitination
K213 Ubiquitination
K261 Ubiquitination
K266 Ubiquitination
S274 Phosphorylation
K277 Ubiquitination
K291 Ubiquitination
K336 Ubiquitination
K342 Ubiquitination
K358 Ubiquitination
R365 Methylation
K368 Ubiquitination
T370 Phosphorylation
Y393 Phosphorylation
S404 Phosphorylation
K412 Ubiquitination
S433 Phosphorylation
K436 Ubiquitination
Y439 Phosphorylation
Y456 Phosphorylation

Research Backgrounds

Function:

A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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