Cytochrome P450 51A1 Antibody | Affinity Biosciences

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Cytochrome P450 51A1 Antibody - Western blot analysis of Cytochrome P450 51A1 using A431 whole cell lysates.

Product:	Cytochrome P450 51A1 Antibody
Catalog:	DF3591
Description:	Rabbit polyclonal antibody to Cytochrome P450 51A1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Dog
Mol.Wt.:	57 KD; 57kD(Calculated).
Uniprot:	Q16850
RRID:	AB_2835963

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

Cytochrome P450 51A1 Antibody detects endogenous levels of total Cytochrome P450 51A1.

RRID:

AB_2835963
Cite Format: Affinity Biosciences Cat# DF3591, RRID:AB_2835963.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CP51; CP51A_HUMAN; CYP51; CYP51A1; CYPL1; CYPLI; Cytochrome P450 51; Cytochrome P450 51A1; Cytochrome P450 family 51 subfamily A member 1; Cytochrome P450 family 51 subfamily A polypeptide 1; Cytochrome P450, 51 (lanosterol 14 alpha demethylase); Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; LDM; P450 14DM; P450L1; Sterol 14-alpha demethylase;

Immunogens

Immunogen:

A synthesized peptide derived from human Cytochrome P450 51A1, corresponding to a region within the internal amino acids.

Uniprot:

Q16850(CP51A_HUMAN) >>Visit HPA database.

Gene(ID):

CYP51A1(1595)

Expression:

Q16850 CP51A_HUMAN:

Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Sequence:

Q16850 CP51A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLLGLLQAGGSVLGQAMEKVTGGNLLSMLLIACAFTLSLVYLIRLAAGHLVQLPAGVKSPPYIFSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFESWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQKKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Horse

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q16850_CP51A_HUMAN

Function:

A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.

Tissue Specificity:

Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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Cytochrome P450 51A1 Antibody - #DF3591