Cytochrome P450 39A1 Antibody - #DF3585
| Product: | Cytochrome P450 39A1 Antibody |
| Catalog: | DF3585 |
| Description: | Rabbit polyclonal antibody to Cytochrome P450 39A1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 54 KD; 54kD(Calculated). |
| Uniprot: | Q9NYL5 |
| RRID: | AB_2835957 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3585, RRID:AB_2835957.
Fold/Unfold
24 hydroxycholesterol 7 alpha hydroxylase; 24-hydroxycholesterol 7-alpha-hydroxylase; CP39A_HUMAN; Cyp39a1; cytochrome P450 family 39, subfamily A polypeptide 1; Cytochrome P450 39A1; cytochrome P450 subfamily XXXIX (oxysterol 7 alpha hydroxylase) polypeptide 1; Cytochrome P450, family 39, subfamily a, polypeptide 1; hCYP39A1; Oxysterol 7 alpha hydroxylase; Oxysterol 7-alpha-hydroxylase; oxysterol 7alpha hydroxylase;
Immunogens
- Q9NYL5 CP39A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELISPTVIIILGCLALFLLLQRKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIKYGPIFTVFAMGNRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRTASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENLGTHGTMDLNNLVRHLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLRNWSKSKKWFLELFEKNIPDIKACKSAKDNSMTLLQATLDIVETETSKENSPNYGLLLLWASLSNAVPVAFWTLAYVLSHPDIHKAIMEGISSVFGKAGKDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWKKANLEKHSFLDCFMAFGSGKFQCPARWFALLEVQMCIILILYKYDCSLLDPLPKQSYLHLVGVPQPEGQCRIEYKQRI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NYL5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y102 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| T245 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot |
Research Backgrounds
A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Endoplasmic reticulum membrane>Multi-pass membrane protein. Microsome membrane>Multi-pass membrane protein.
Liver specific.
Belongs to the cytochrome P450 family.
Research Fields
· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.