Cytochrome P450 2E1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat-shock treatment), using Cytochrome P450 2E1 Antibody.

DF3578 at 1/100 staining Mouse liver tissue by IHC-P.

DF3578 at 1/100 staining Rat liver tissue by IHC-P.

Product:	Cytochrome P450 2E1 Antibody
Catalog:	DF3578
Description:	Rabbit polyclonal antibody to Cytochrome P450 2E1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	57 KD; 57kD(Calculated).
Uniprot:	P05181
RRID:	AB_2835950

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Cytochrome P450 2E1 Antibody detects endogenous levels of total Cytochrome P450 2E1.

RRID:

AB_2835950
Cite Format: Affinity Biosciences Cat# DF3578, RRID:AB_2835950.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE1; CYP2E; Cyp2e1; CYPIIE1; Cytochrome P450 2E1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol-inducible P450; Flavoprotein-linked monooxygenase; MGC133529; Microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450-J; P450C2E; P450J; P450RLM6; Xenobiotic monooxygenase;

Immunogens

Immunogen:

Uniprot:

P05181(CP2E1_HUMAN) >>Visit HPA database.

Gene(ID):

CYP2E1(1571)

Sequence:

P05181 CP2E1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSALGVTVALLVWAAFLLLVSMWRQVHSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGPTWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Xenopus

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05181 As Substrate

P05181

Site	PTM Type	Source
S56	Phosphorylation	Uniprot
T58	Phosphorylation	Uniprot
T69	Phosphorylation	Uniprot
S74	Phosphorylation	Uniprot
Y83	Phosphorylation	Uniprot
K84	Ubiquitination	Uniprot
K87	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot
T121	Phosphorylation	Uniprot
S129	Phosphorylation	Uniprot
T131	Phosphorylation	Uniprot
T132	Phosphorylation	Uniprot
S145	Phosphorylation	Uniprot
Y245	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
K251	Ubiquitination	Uniprot
S256	Phosphorylation	Uniprot
K275	Ubiquitination	Uniprot
T373	Phosphorylation	Uniprot
T376	Phosphorylation	Uniprot
T387	Phosphorylation	Uniprot
K410	Ubiquitination	Uniprot
K420	Ubiquitination	Uniprot
K422	Ubiquitination	Uniprot
S424	Phosphorylation	Uniprot
K428	Ubiquitination	Uniprot
S431	Phosphorylation	Uniprot
T432	Phosphorylation	Uniprot
K434	Ubiquitination	Uniprot
K461	Ubiquitination	Uniprot

Research Backgrounds

P05181_CP2E1_HUMAN

Function:

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein. Mitochondrion inner membrane>Peripheral membrane protein.
Note: Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.

Subunit Structure:

Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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Cytochrome P450 2E1 Antibody - #DF3578