Cytochrome P450 2C8 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse testis, using Cytochrome P450 2C8 Antibody at 1/1000 dilution.

Product:	Cytochrome P450 2C8 Antibody
Catalog:	DF3575
Description:	Rabbit polyclonal antibody to Cytochrome P450 2C8
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	56kD; 56kD(Calculated).
Uniprot:	P10632
RRID:	AB_2835947

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Cytochrome P450 2C8 Antibody detects endogenous levels of total Cytochrome P450 2C8.

RRID:

AB_2835947
Cite Format: Affinity Biosciences Cat# DF3575, RRID:AB_2835947.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CP2C8_HUMAN; CPC8; CYP2C8; CYPIIC8; Cytochrome P450 2C8; Cytochrome P450 family 2 subfamily C polypeptide 8; Cytochrome P450 form 1; Cytochrome P450 IIC2; Cytochrome P450 MP-12; Cytochrome P450 MP-20; Cytochrome P450 subfamily IIC (mephenytoin 4 hydroxylase) polypeptide 8; Flavoprotein linked monooxygenase; Microsomal monooxygenase; MP 12/MP 20; P450 form 1; P450 IIC2; P450 MP 12/MP 20; S mephenytoin 4 hydroxylase; S-mephenytoin 4-hydroxylase; Xenobiotic monooxygenase;

Immunogens

Immunogen:

Uniprot:

P10632(CP2C8_HUMAN) >>Visit HPA database.

Gene(ID):

CYP2C8(1558)

Sequence:

P10632 CP2C8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPFVVLVLCLSFMLLFSLWRQSCRRRKLPPGPTPLPIIGNMLQIDVKDICKSFTNFSKVYGPVFTVYFGMNPIVVFHGYEAVKEALIDNGEEFSGRGNSPISQRITKGLGIISSNGKRWKEIRRFSLTTLRNFGMGKRSIEDRVQEEAHCLVEELRKTKASPCDPTFILGCAPCNVICSVVFQKRFDYKDQNFLTLMKRFNENFRILNSPWIQVCNNFPLLIDCFPGTHNKVLKNVALTRSYIREKVKEHQASLDVNNPRDFIDCFLIKMEQEKDNQKSEFNIENLVGTVADLFVAGTETTSTTLRYGLLLLLKHPEVTAKVQEEIDHVIGRHRSPCMQDRSHMPYTDAVVHEIQRYSDLVPTGVPHAVTTDTKFRNYLIPKGTTIMALLTSVLHDDKEFPNPNIFDPGHFLDKNGNFKKSDYFMPFSAGKRICAGEGLARMELFLFLTTILQNFNLKSVDDLKNLNTTAVTKGIVSLPPSYQICFIPV

PTMs - P10632 As Substrate

P10632

Site	PTM Type	Source
S100	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
T129	Phosphorylation	Uniprot
S254	Phosphorylation	Uniprot
C435	S-Nitrosylation	Uniprot

Research Backgrounds

P10632_CP2C8_HUMAN

Function:

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Primarily catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) with a preference for the last double bond. Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes all trans-retinoic acid toward its 4-hydroxylated form. Displays 16-alpha hydroxylase activity toward estrogen steroid hormones, 17beta-estradiol (E2) and estrone (E1). Plays a role in the oxidative metabolism of xenobiotics. It is the principal enzyme responsible for the metabolism of the anti-cancer drug paclitaxel (taxol).

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Serotonergic synapse.

Restrictive clause

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Cytochrome P450 2C8 Antibody - #DF3575