GALK1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293, using GALK1 Antibody.

Product:	GALK1 Antibody
Catalog:	AF0367
Description:	Rabbit polyclonal antibody to GALK1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	45kDa; 42kD(Calculated).
Uniprot:	P51570
RRID:	AB_2833531

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

GALK1 Antibody detects endogenous levels of total GALK1.

RRID:

AB_2833531
Cite Format: Affinity Biosciences Cat# AF0367, RRID:AB_2833531.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.7.1.6; epididymis secretory protein Li 19; Galactokinase 1; Galactokinase; Galactose kinase; GALK 1; GALK; GALK1; GALK1_HUMAN; GK1; GLK; GLK1; HEL-S-19;

Immunogens

Immunogen:

Uniprot:

P51570(GALK1_HUMAN) >>Visit HPA database.

Gene(ID):

GALK1(2584)

Description:

GALK1 Major enzyme for galactose metabolism. Belongs to the GHMP kinase family. GalK subfamily.

Sequence:

P51570 GALK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALRQPQVAELLAEARRAFREEFGAEPELAVSAPGRVNLIGEHTDYNQGLVLPMALELMTVLVGSPRKDGLVSLLTTSEGADEPQRLQFPLPTAQRSLEPGTPRWANYVKGVIQYYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATYTFLQQLCPDSGTIAARAQVCQQAEHSFAGMPCGIMDQFISLMGQKGHALLIDCRSLETSLVPLSDPKLAVLITNSNVRHSLASSEYPVRRRQCEEVARALGKESLREVQLEELEAARDLVSKEGFRRARHVVGEIRRTAQAAAALRRGDYRAFGRLMVESHRSLRDDYEVSCPELDQLVEAALAVPGVYGSRMTGGGFGGCTVTLLEASAAPHAMRHIQEHYGGTATFYLSQAADGAKVLCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51570 As Substrate

P51570

Site	PTM Type	Source
T61	Phosphorylation	Uniprot
S66	Phosphorylation	Uniprot
K69	Ubiquitination	Uniprot
K217	Ubiquitination	Uniprot
S230	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
Y236	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
K272	Ubiquitination	Uniprot

Research Backgrounds

P51570_GALK1_HUMAN

Function:

Major enzyme for galactose metabolism.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the GHMP kinase family. GalK subfamily.

Research Fields

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GALK1 Antibody - #AF0367