Product: Cytochrome P450 24A1 Antibody
Catalog: DF3568
Description: Rabbit polyclonal antibody to Cytochrome P450 24A1
Application: WB IF/ICC
Reactivity: Human
Prediction: Rabbit, Dog
Mol.Wt.: 60 KD; 59kD(Calculated).
Uniprot: Q07973
RRID: AB_2835940

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Rabbit(92%), Dog(83%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 24A1 Antibody detects endogenous levels of total Cytochrome P450 24A1.
RRID:
AB_2835940
Cite Format: Affinity Biosciences Cat# DF3568, RRID:AB_2835940.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1 25 @dihydroxyvitamin D3 24 hydroxylase; 1 25 dihydroxyvitamin D(3) 24 hydroxylase; 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24 OHase; 24-OHase; 25-dihydroxyvitamin D(3) 24-hydroxylase; CP 24; CP24; CP24A_HUMAN; CYP 24; CYP24; CYP24A1; Cytochrome P450 24A1; Cytochrome P450 24A1 mitochondrial; cytochrome P450 CC24; Cytochrome P450 family 24; Cytochrome P450 family 24 subfamily A member 1; Cytochrome P450 family 24 subfamily A polypeptide 1; Cytochrome P450 subfamily XXIV; Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; EC 1.14.13.n4; Exo mitochondrial protein; HCAI; HCINF1; MGC126273; MGC126274; mitochondrial; P450 CC24; Vitamin D 24 hydroxylase; Vitamin D(3) 24 hydroxylase; Vitamin D(3) 24-hydroxylase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSSPISKSRSLAAFLQQLRSPRQPPRLVTSTAYTSPQPREVPVCPLTAGGETQNAAALPGPTSWPLLGSLLQILWKGGLKKQHDTLVEYHKKYGKIFRMKLGSFESVHLGSPCLLEALYRTESAYPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSAFQKKLMKPGEVMKLDNKINEVLADFMGRIDELCDERGHVEDLYSELNKWSFESICLVLYEKRFGLLQKNAGDEAVNFIMAIKTMMSTFGRMMVTPVELHKSLNTKVWQDHTLAWDTIFKSVKACIDNRLEKYSQQPSADFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Dog
83
Horse
75
Sheep
75
Chicken
50
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07973 As Substrate

Site PTM Type Enzyme
S20 Phosphorylation
S35 Phosphorylation
Y125 Phosphorylation
K165 Acetylation
Y220 Phosphorylation
T388 Phosphorylation
T395 Phosphorylation
K400 Ubiquitination
Y407 Phosphorylation
S504 Phosphorylation

Research Backgrounds

Function:

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). High sodium reduced the expression of PTH1R and Klotho by inhibiting 1,25(OH)2D3 synthesis in cultured proximal tubule epithelial cells. Annals of Translational Medicine, 2022 (PubMed: 35928745)

Application: WB    Species: Human    Sample: HK-2 cells

Figure 2 High sodium reduced the expression of 24-OHase (Cyp24a1) in HK-2 cells. (A,B) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in different concentrations of sodium (140/150/160/170 mM) for 24 hours. (C,D) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in different concentrations of phosphate (0.93/2/3/4 mM) for 24 hours. (E,F) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in N, HP (4 mM), HNa (170 mM) and M with the same osmotic pressure for 24 hours. a, versus N,

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.