Cytochrome P450 24A1 Antibody | Affinity Biosciences

Cites

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Figure 2 High sodium reduced the expression of 24-OHase (Cyp24a1) in HK-2 cells.

Product:	Cytochrome P450 24A1 Antibody
Catalog:	DF3568
Description:	Rabbit polyclonal antibody to Cytochrome P450 24A1
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rabbit, Dog
Mol.Wt.:	60 KD; 59kD(Calculated).
Uniprot:	Q07973
RRID:	AB_2835940

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

Cytochrome P450 24A1 Antibody detects endogenous levels of total Cytochrome P450 24A1.

RRID:

AB_2835940
Cite Format: Affinity Biosciences Cat# DF3568, RRID:AB_2835940.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1 25 @dihydroxyvitamin D3 24 hydroxylase; 1 25 dihydroxyvitamin D(3) 24 hydroxylase; 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24 OHase; 24-OHase; 25-dihydroxyvitamin D(3) 24-hydroxylase; CP 24; CP24; CP24A_HUMAN; CYP 24; CYP24; CYP24A1; Cytochrome P450 24A1; Cytochrome P450 24A1 mitochondrial; cytochrome P450 CC24; Cytochrome P450 family 24; Cytochrome P450 family 24 subfamily A member 1; Cytochrome P450 family 24 subfamily A polypeptide 1; Cytochrome P450 subfamily XXIV; Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; EC 1.14.13.n4; Exo mitochondrial protein; HCAI; HCINF1; MGC126273; MGC126274; mitochondrial; P450 CC24; Vitamin D 24 hydroxylase; Vitamin D(3) 24 hydroxylase; Vitamin D(3) 24-hydroxylase;

Immunogens

Immunogen:

Uniprot:

Q07973(CP24A_HUMAN) >>Visit HPA database.

Gene(ID):

CYP24A1(1591)

Sequence:

Q07973 CP24A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSPISKSRSLAAFLQQLRSPRQPPRLVTSTAYTSPQPREVPVCPLTAGGETQNAAALPGPTSWPLLGSLLQILWKGGLKKQHDTLVEYHKKYGKIFRMKLGSFESVHLGSPCLLEALYRTESAYPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSAFQKKLMKPGEVMKLDNKINEVLADFMGRIDELCDERGHVEDLYSELNKWSFESICLVLYEKRFGLLQKNAGDEAVNFIMAIKTMMSTFGRMMVTPVELHKSLNTKVWQDHTLAWDTIFKSVKACIDNRLEKYSQQPSADFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQKLLKEIQSVLPENQVPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKATVLGEYALPKGTVLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Horse

Sheep

Chicken

Pig

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07973 As Substrate

Q07973

Site	PTM Type	Source
S20	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
Y125	Phosphorylation	Uniprot
K165	Acetylation	Uniprot
Y220	Phosphorylation	Uniprot
T388	Phosphorylation	Uniprot
T395	Phosphorylation	Uniprot
K400	Ubiquitination	Uniprot
Y407	Phosphorylation	Uniprot
S504	Phosphorylation	Uniprot

Research Backgrounds

Q07973_CP24A_HUMAN

Function:

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.

Subcellular Location:

Mitochondrion.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). High sodium reduced the expression of PTH1R and Klotho by inhibiting 1,25(OH)2D3 synthesis in cultured proximal tubule epithelial cells. Annals of Translational Medicine, 2022 (PubMed: 35928745)

Application: WB Species: Human Sample: HK-2 cells

Figure 2 High sodium reduced the expression of 24-OHase (Cyp24a1) in HK-2 cells. (A,B) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in different concentrations of sodium (140/150/160/170 mM) for 24 hours. (C,D) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in different concentrations of phosphate (0.93/2/3/4 mM) for 24 hours. (E,F) mRNA and protein expression of Cyp24a1 in HK-2 cells after incubation in N, HP (4 mM), HNa (170 mM) and M with the same osmotic pressure for 24 hours. a, versus N,

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Cytochrome P450 24A1 Antibody - #DF3568