Collagen VI alpha 2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat brain, using Collagen VI α2 antibody.

Western blot analysis of extracts from various samples, using Collagen VI alpha 2 Antibody.

Product:	Collagen VI alpha 2 Antibody
Catalog:	DF3552
Description:	Rabbit polyclonal antibody to Collagen VI alpha 2
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	109 KD; 109kD(Calculated).
Uniprot:	P12110
RRID:	AB_2835925

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

Collagen VI alpha 2 Antibody detects endogenous levels of total Collagen VI alpha 2.

RRID:

AB_2835925
Cite Format: Affinity Biosciences Cat# DF3552, RRID:AB_2835925.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CO6A2_HUMAN; COL6A2; Collagen alpha 2(VI) chain; Collagen alpha-2(VI) chain; collagen type VI alpha 2; Collagen VI alpha 2 polypeptide; human mRNA for collagen VI alpha 2 C terminal globular domain; PP3610;

Immunogens

Immunogen:

Uniprot:

P12110(CO6A2_HUMAN) >>Visit HPA database.

Gene(ID):

COL6A2(1292)

Sequence:

P12110 CO6A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLQGTCSVLLLWGILGAIQAQQQEVISPDTTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFRRGTFTDCALANMTEQIRQDRSKGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQGLRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPSGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDPGNRGPDGYPGEAGSPGERGDQGGKGDPGRPGRRGPPGEIGAKGSKGYQGNSGAPGSPGVKGAKGGPGPRGPKGEPGRRGDPGTKGSPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGEKGEPGPRGPEGGRGDFGLKGEPGRKGEKGEPADPGPPGEPGPRGPRGVPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYDRLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLARRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGGARRHAELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIRWIC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12110 As Substrate

P12110

Site	PTM Type	Source
K120	Ubiquitination	Uniprot
S126	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
N140	N-Glycosylation	Uniprot
K197	Ubiquitination	Uniprot
T207	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
K393	Ubiquitination	Uniprot
S399	Phosphorylation	Uniprot
Y597	Phosphorylation	Uniprot
K609	Acetylation	Uniprot
Y665	Phosphorylation	Uniprot
S666	Phosphorylation	Uniprot
S683	Phosphorylation	Uniprot
S685	Phosphorylation	Uniprot
K688	Ubiquitination	Uniprot
K692	Ubiquitination	Uniprot
T701	Phosphorylation	Uniprot
T703	Phosphorylation	Uniprot
S705	Phosphorylation	Uniprot
N785	N-Glycosylation	Uniprot
K851	Ubiquitination	Uniprot
N897	N-Glycosylation	Uniprot
N954	N-Glycosylation	Uniprot
K1000	Ubiquitination	Uniprot

Research Backgrounds

P12110_CO6A2_HUMAN

Function:

Collagen VI acts as a cell-binding protein.

PTMs:

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Membrane>Peripheral membrane protein.
Note: Recruited on membranes by CSPG4.

Subunit Structure:

Trimers composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). Interacts with CSPG4.

Family&Domains:

Belongs to the type VI collagen family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB). Clinical Proteomics, 2019 (PubMed: 30988666) [IF=3.8]

Application: WB Species: human Sample: ESCC tissues

Fig. 5|The diferentially expressed proteins were validated by Western Blot. Compared with adjacent normal tissues, the protein expression of PTMA, PAK2, PPP1CA, HMGB2 were up-regulated (a, b), and the protein expression of Caveolin, Integrin beta-1, Collagen alpha-2(VI), Leiomodin-1, Vinculin were down-regulated in ESCC tissues from four pairs of samples (c, d). Representative immunoblot images (a, c) and histograms (mean ± SD; b, d).The experiments were repeated at least three times, N represented normal tissues and T represented tumor tissues

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Collagen VI alpha 2 Antibody - #DF3552