CDKAL Antibody - #DF3546
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3546, RRID:AB_2835919.
Fold/Unfold
CDK5 regulatory subunit associated protein 1 like 1; CDK5 regulatory subunit-associated protein 1-like 1; CDKAL_HUMAN; CDKAL1; Threonylcarbamoyladenosine tRNA methylthiotransferase; tRNA t 6 A37 methylthiotransferase; tRNA-t(6)A37 methylthiotransferase;
Immunogens
- Q5VV42 CDKAL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSASCDTLLDDIEDIVSQEDSKPQDRHFVRKDVVPKVRRRNTQKYLQEEENSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVDRAKQSFQEGVCEIWLTSEDTGAYGRDIGTNLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMEMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKMEQVPAQVKKQRTKDLSRVFHSYSPYDHKIGERQQVLVTEESFDSKFYVAHNQFYEQVLVPKNPAFMGKMVEVDIYESGKHFMKGQPVSDAKVYTPSISKPLAKGEVSGLTKDFRNGLGNQLSSGSHTSAASQCDSASSRMVLPMPRLHQDCALRMSVGLALLGLLFAFFVKVYN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5VV42 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K37 | Ubiquitination | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Endoplasmic reticulum membrane>Single-pass membrane protein.
Note: Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
Expressed in pancreatic islets.
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
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For Research Use Only.
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