CDCA2 Antibody - #DF3543

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Product: CDCA2 Antibody
Catalog: DF3543
Description: Rabbit polyclonal antibody to CDCA2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 113,140kD; 113kD(Calculated).
Uniprot: Q69YH5
RRID: AB_2835916

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(90%), Sheep(91%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CDCA2 Antibody detects endogenous levels of total CDCA2.
RRID:
AB_2835916
Cite Format: Affinity Biosciences Cat# DF3543, RRID:AB_2835916.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cell division cycle associated 2; PPP1R81; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man; protein phosphatase 1, regulatory subunit 81; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo Man; Repo-Man;

Immunogens

Immunogen:
Uniprot:

Q69YH5(CDCA2_HUMAN) >>Visit HPA database.

Gene(ID):
CDCA2(157313)
Expression:
Q69YH5 CDCA2_HUMAN:

Ubiquitously expressed.

Sequence:
MDANSKDKPPETKESAMNNAGNASFILGTGKIVTPQKHAELPPNPCTPDTFKSPLNFSTVTVEQLGITPESFVRNSAGKSSSYLKKCRRRSAVGARGSPETNHLIRFIARQQNIKNARKSPLAQDSPSQGSPALYRNVNTLRERISAFQSAFHSIKENEKMTGCLEFSEAGKESEMTDLTRKEGLSACQQSGFPAVLSSKRRRISYQRDSDENLTDAEGKVIGLQIFNIDTDRACAVETSVDLSEISSKLGSTQSGFLVEESLPLSELTETSNALKVADCVVGKGSSDAVSPDTFTAEVSSDAVPDVRSPATPACRRDLPTPKTFVLRSVLKKPSVKMCLESLQEHCNNLYDDDGTHPSLISNLPNCCKEKEAEDEENFEAPAFLNMRKRKRVTFGEDLSPEVFDESLPANTPLRKGGTPVCKKDFSGLSSLLLEQSPVPEPLPQPDFDDKGENLENIEPLQVSFAVLSSPNKSSISETLSGTDTFSSSNNHEKISSPKVGRITRTSNRRNQLVSVVEESVCNLLNTEVQPCKEKKINRRKSQETKCTKRALPKKSQVLKSCRKKKGKGKKSVQKSLYGERDIASKKPLLSPIPELPEVPEMTPSIPSIRRLGSGYFSSNGKLEEVKTPKNPVKRKDLLRHDPDLHMHQGYDKYDVSEFCSYIKSSSSLGNATSDEDPNTNIMNINENKNIPKAKNKSESENEPKAGTDSPVSCASVTEERVASDSPKPALTLQQGQEFSAGGQNAENLCQFFKISPDLNIKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSEDLGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSSSVGSSVEISLENSELFKDLSDAIEQTFQRRNSETKVRRSTRLQKDLENEGLVWISLPLPSTSQKAKRRTICTFDSSGFESMSPIKETVSSRQKPQMAPPVSDPENSQGPAAGSSDEPGKRRKSFCISTLANTKATSQFKGYRRRSSLNGKGESSLTALERIEHNGERKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Bovine
91
Sheep
91
Dog
91
Horse
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q69YH5 As Substrate

Site PTM Type Enzyme
K6 Methylation
K13 Methylation
S24 Phosphorylation
T47 Phosphorylation
K52 Methylation
S53 Phosphorylation
T59 Phosphorylation
T68 Phosphorylation
S76 Phosphorylation
K79 Ubiquitination
S81 Phosphorylation
S91 Phosphorylation
R96 Methylation
S98 Phosphorylation
T101 Phosphorylation
R106 Methylation
K115 Acetylation
K119 Acetylation
K119 Methylation
K119 Ubiquitination
S120 Phosphorylation
S126 Phosphorylation
S128 Phosphorylation
S131 Phosphorylation
Y135 Phosphorylation
K182 Ubiquitination
S186 Phosphorylation
S191 Phosphorylation
K200 Ubiquitination
S205 Phosphorylation
S210 Phosphorylation
K276 Ubiquitination
K284 Ubiquitination
S286 Phosphorylation
S291 Phosphorylation
S301 Phosphorylation
S309 Phosphorylation
T312 Phosphorylation
K323 Ubiquitination
T324 Phosphorylation
S329 Phosphorylation
T394 Phosphorylation
S400 Phosphorylation
S407 Phosphorylation
T412 Phosphorylation
K424 Ubiquitination
S437 Phosphorylation
S470 Phosphorylation
K494 Ubiquitination
S497 Phosphorylation
S507 Phosphorylation
S556 Phosphorylation
K575 Ubiquitination
S591 Phosphorylation
T603 Phosphorylation
S608 Phosphorylation
S614 Phosphorylation
K622 Acetylation
K622 Sumoylation
K627 Acetylation
T628 Phosphorylation
S710 Phosphorylation
C714 S-Nitrosylation
T718 Phosphorylation
S724 Phosphorylation
S726 Phosphorylation
S756 Phosphorylation
K762 Sumoylation
K766 Ubiquitination
K776 Ubiquitination
R813 Methylation
S833 Phosphorylation
S874 Phosphorylation
S886 Phosphorylation
S893 Phosphorylation Q96GD4 (AURKB)
S930 Phosphorylation
S936 Phosphorylation
S955 Phosphorylation
S960 Phosphorylation
S977 Phosphorylation
S1000 Phosphorylation

Research Backgrounds

Function:

Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.

PTMs:

Phosphorylated by CDK1. May regulate its subcellular location.

Subcellular Location:

Nucleus.
Note: Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with PPP1CC.

References

1). Cell division cycle associated 2 (CDCA2) upregulation promotes the progression of hepatocellular carcinoma in a p53-dependant manner. PeerJ, 2017 (PubMed: 35694386) [IF=2.7]

Application: WB    Species: Human    Sample: liver tissues

Figure 1 Expression of CDCA2 in hepatocellular carcinoma. Analysis of an RNA-seq dataset from UALCAN showed (A) elevated CDCA2 expression in primary HCC tissues compared with normal liver tissues; (B) increased CDCA2 levels with clinical stage; (C) elevated CDCA2 expression in samples expressing mutant TP53 compared with samples expressing wild-type TP53; (D) worse survival of patients with high CDCA2 expression than in patients with low CDCA2 expression; and (E) worse survival of patients with high CDCA2 expression than patients with low CDCA2 expression among Asian patients but not among White Caucasian patients. (F) Our study found elevated CDCA2 mRNA levels in 40 HCC tissues compared to paired normal liver tissues. (G) Representative photos of +ve and –ve immunohistochemistry staining of CDCA2 in HCC or normal liver tissues. (H) qRT-PCR measured CDCA2 mRNA expression in the L02 and HCC cell lines. (I) Western blotting analysis of CDCA2 protein expression in the L02 and HCC cell lines. Stars indicate the P value of the CDCA2 protein level compared with that in the L02 cells. (J) Western blotting analysis of CDCA2 protein expression in HCC cell lines with lentivirus-mediated stable CDCA2 knockdown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

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