Cytochrome c-type Heme Lyase Antibody

WB
IF/ICC

1/2

Western blot analysis of extracts from rat kidney, using Cytochrome c-type Heme Lyase Antibody.

Product:	Cytochrome c-type Heme Lyase Antibody
Catalog:	DF3540
Description:	Rabbit polyclonal antibody to Cytochrome c-type Heme Lyase
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	31 KD; 31kD(Calculated).
Uniprot:	P53701
RRID:	AB_2835913

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

Cytochrome c-type Heme Lyase Antibody detects endogenous levels of total Cytochrome c-type Heme Lyase.

RRID:

AB_2835913
Cite Format: Affinity Biosciences Cat# DF3540, RRID:AB_2835913.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCHL; CCHL_HUMAN; cytochrome c heme-lyase; Cytochrome c-type heme lyase; DKFZp779I1858; EC 4.4.1.17; Hccs; Holocytochrome c synthase (cytochrome c heme lyase); Holocytochrome c synthase; Holocytochrome c type synthase; Holocytochrome c-type synthase; MCOPS7; OTTHUMP00000022903; OTTHUMP00000022904; OTTHUMP00000022905; OTTMUSP00000021173; OTTMUSP00000021174; RGD1563855; RP23-37L2.1;

Immunogens

Immunogen:

Uniprot:

P53701(CCHL_HUMAN) >>Visit HPA database.

Gene(ID):

HCCS(3052)

Sequence:

P53701 CCHL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53701 As Substrate

P53701

Site	PTM Type	Source
G2	Myristoylation	Uniprot
T39	Phosphorylation	Uniprot
K49	Ubiquitination	Uniprot
Y61	Phosphorylation	Uniprot
Y63	Phosphorylation	Uniprot
K76	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K138	Ubiquitination	Uniprot
K145	Sumoylation	Uniprot
K145	Ubiquitination	Uniprot
K163	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
S200	Phosphorylation	Uniprot
Y224	Phosphorylation	Uniprot
Y228	Phosphorylation	Uniprot
Y229	Phosphorylation	Uniprot
T241	Phosphorylation	Uniprot

Research Backgrounds

P53701_CCHL_HUMAN

Function:

Links covalently the heme group to the apoprotein of cytochrome c.

Subcellular Location:

Mitochondrion inner membrane. Membrane>Lipid-anchor.

Family&Domains:

Belongs to the cytochrome c-type heme lyase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

Restrictive clause

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For Research Use Only.
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Cytochrome c-type Heme Lyase Antibody - #DF3540