Product: RPS4X Antibody
Catalog: DF3685
Description: Rabbit polyclonal antibody to RPS4X
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 30 KD; 30kD(Calculated).
Uniprot: P62701
RRID: AB_2835895

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
RPS4X Antibody detects endogenous levels of total RPS4X.
RRID:
AB_2835895
Cite Format: Affinity Biosciences Cat# DF3685, RRID:AB_2835895.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S4; CCG2; Cell cycle gene 2; DXS306; FLJ40595; ribosomal protein S4, X-linked; Ribosomal protein S4X isoform; RPS4; rps4x; RS4X_HUMAN; S4; SCAR; SCR10; Single copy abundant mRNA protein; Single-copy abundant mRNA; X isoform;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
89
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62701 As Substrate

Site PTM Type Enzyme
K7 Methylation
K16 Acetylation
K16 Ubiquitination
K22 Acetylation
K22 Ubiquitination
T24 Phosphorylation
S32 Phosphorylation
T33 Phosphorylation
K37 Ubiquitination
C41 S-Nitrosylation
K53 Acetylation
K53 Methylation
K53 Ubiquitination
Y54 Phosphorylation
T57 Phosphorylation
K62 Acetylation
K62 Ubiquitination
K63 Ubiquitination
K71 Ubiquitination
K75 Ubiquitination
T78 Phosphorylation
S91 Phosphorylation
K94 Ubiquitination
Y103 Phosphorylation
T105 Phosphorylation
K106 Ubiquitination
K120 Acetylation
K120 Ubiquitination
Y121 Phosphorylation
K125 Ubiquitination
K128 Ubiquitination
T133 Phosphorylation
K134 Acetylation
K134 Ubiquitination
R145 Methylation
R148 Methylation
K168 Ubiquitination
T177 Phosphorylation
C181 S-Nitrosylation
T184 Phosphorylation
T196 Phosphorylation
R198 Methylation
R200 Methylation
S204 Phosphorylation
K211 Ubiquitination
S217 Phosphorylation
S223 Phosphorylation
K230 Ubiquitination
K233 Acetylation
K233 Ubiquitination
S237 Phosphorylation
T247 Phosphorylation
K259 Ubiquitination

Research Backgrounds

Subcellular Location:

Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.

Family&Domains:

Belongs to the eukaryotic ribosomal protein eS4 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.