RPS23 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using RPS23 Antibody.

Product:	RPS23 Antibody
Catalog:	DF3682
Description:	Rabbit polyclonal antibody to RPS23
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	16 KD; 16kD(Calculated).
Uniprot:	P62266
RRID:	AB_2835892

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

RPS23 Antibody detects endogenous levels of total RPS23.

RRID:

AB_2835892
Cite Format: Affinity Biosciences Cat# DF3682, RRID:AB_2835892.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

40S ribosomal protein S23; FLJ35016; Homolog of yeast ribosomal protein S28; Ribosomal protein S23; RPS 23; RPS23; RS23_HUMAN; S23;

Immunogens

Immunogen:

Uniprot:

P62266(RS23_HUMAN) >>Visit HPA database.

Gene(ID):

RPS23(6228)

Sequence:

P62266 RS23_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62266 As Substrate

P62266

Site	PTM Type	Source
K25	Ubiquitination	Uniprot
K29	Sumoylation	Uniprot
K29	Ubiquitination	Uniprot
K37	Sumoylation	Uniprot
K37	Ubiquitination	Uniprot
K48	Sumoylation	Uniprot
K48	Ubiquitination	Uniprot
K54	Sumoylation	Uniprot
K54	Ubiquitination	Uniprot
K60	Ubiquitination	Uniprot
K76	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
K108	Ubiquitination	Uniprot
S129	Phosphorylation	Uniprot
Y134	Phosphorylation	Uniprot
K135	Acetylation	Uniprot

Research Backgrounds

P62266_RS23_HUMAN

Function:

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays an important role in translational accuracy.

PTMs:

Hydroxylation at Pro-62 affects translation termination efficiency.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm. Rough endoplasmic reticulum.
Note: Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity).

Subunit Structure:

Component of the 40S small ribosomal subunit.

Family&Domains:

Belongs to the universal ribosomal protein uS12 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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RPS23 Antibody - #DF3682