Product: RPS20 Antibody
Catalog: DF3681
Description: Rabbit polyclonal antibody to RPS20
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 13 KD; 13kD(Calculated).
Uniprot: P60866
RRID: AB_2835891

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPS20 Antibody detects endogenous levels of total RPS20.
RRID:
AB_2835891
Cite Format: Affinity Biosciences Cat# DF3681, RRID:AB_2835891.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40S ribosomal protein S20; Ribosomal protein S20; RPS 20; RpS20; RS20_HUMAN; S20;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60866 As Substrate

Site PTM Type Enzyme
K4 Acetylation
K4 Ubiquitination
T6 Phosphorylation
K8 Acetylation
K8 Methylation
K8 Sumoylation
K8 Ubiquitination
T9 Phosphorylation
R19 Methylation
K30 Ubiquitination
S31 Phosphorylation
K34 Acetylation
K34 Ubiquitination
R41 Methylation
K44 Acetylation
K44 Ubiquitination
K49 Ubiquitination
K51 Ubiquitination
T58 Phosphorylation
K59 Ubiquitination
K67 Ubiquitination
K75 Ubiquitination
T76 Phosphorylation
R79 Methylation
S93 Phosphorylation
S95 Phosphorylation
K99 Ubiquitination

Research Backgrounds

PTMs:

Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the 40S small ribosomal subunit.

Family&Domains:

Belongs to the universal ribosomal protein uS10 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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