CDH3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from 293, using CDH3 Antibody.

Product:	CDH3 Antibody
Catalog:	DF3531
Description:	Rabbit polyclonal antibody to CDH3
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	86 KD; 91kD(Calculated).
Uniprot:	P22223
RRID:	AB_2835885

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CDH3 Antibody detects endogenous levels of total CDH3.

RRID:

AB_2835885
Cite Format: Affinity Biosciences Cat# DF3531, RRID:AB_2835885.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CADH3_HUMAN; Cadherin 3; Cadherin 3 precursor; Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; P cadherin (placental); P-cadherin; PCAD; Placental cadherin;

Immunogens

Immunogen:

Uniprot:

P22223(CADH3_HUMAN) >>Visit HPA database.

Gene(ID):

CDH3(1001)

Expression:

P22223 CADH3_HUMAN:

Expressed in some normal epithelial tissues and in some carcinoma cell lines.

Sequence:

P22223 CADH3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLPRGPLASLLLLQVCWLQCAASEPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQVTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRLTVTDLDAPNSPAWRATYLIMGGDDGDHFTITTHPESNQGILTTRKGLDFEAKNQHTLYVEVTNEAPFVLKLPTSTATIVVHVEDVNEAPVFVPPSKVVEVQEGIPTGEPVCVYTAEDPDKENQKISYRILRDPAGWLAMDPDSGQVTAVGTLDREDEQFVRNNIYEVMVLAMDNGSPPTTGTGTLLLTLIDVNDHGPVPEPRQITICNQSPVRQVLNITDKDLSPHTSPFQAQLTDDSDIYWTAEVNEEGDTVVLSLKKFLKQDTYDVHLSLSDHGNKEQLTVIRATVCDCHGHVETCPGPWKGGFILPVLGAVLALLFLLLVLLLLVRKKRKIKEPLLLPEDDTRDNVFYYGEEGGGEEDQDYDITQLHRGLEARPEVVLRNDVAPTIIPTPMYRPRPANPDEIGNFIIENLKAANTDPTAPPYDTLLVFDYEGSGSDAASLSSLTSSASDQDQDYDYLNEWGSRFKKLADMYGGGEDD

PTMs - P22223 As Substrate

P22223

Site	PTM Type	Source
S115	Phosphorylation	Uniprot
S133	Phosphorylation	Uniprot
T139	Phosphorylation	Uniprot
Y143	Phosphorylation	Uniprot
S359	Phosphorylation	Uniprot
S492	Phosphorylation	Uniprot
K682	Ubiquitination	Uniprot
K684	Ubiquitination	Uniprot
T694	Phosphorylation	Uniprot
Y700	Phosphorylation	Uniprot
Y701	Phosphorylation	Uniprot
Y713	Phosphorylation	Uniprot
T741	Phosphorylation	Uniprot
K818	Ubiquitination	Uniprot
Y823	Phosphorylation	Uniprot

Research Backgrounds

P22223_CADH3_HUMAN

Function:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed in some normal epithelial tissues and in some carcinoma cell lines.

Subunit Structure:

Interacts with CDCP1 and CTNNB1.

Family&Domains:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

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For Research Use Only.
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CDH3 Antibody - #DF3531