ADRB1 Antibody - #DF3511

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Product: ADRB1 Antibody
Catalog: DF3511
Description: Rabbit polyclonal antibody to ADRB1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep
Mol.Wt.: 51 KD; 51kD(Calculated).
Uniprot: P08588
RRID: AB_2835873

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(89%), Sheep(89%)
Clonality:
Polyclonal
Specificity:
ADRB1 Antibody detects endogenous levels of total ADRB1.
RRID:
AB_2835873
Cite Format: Affinity Biosciences Cat# DF3511, RRID:AB_2835873.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1; B1AR; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; RHR;

Immunogens

Immunogen:
Uniprot:

P08588(ADRB1_HUMAN) >>Visit HPA database.

Gene(ID):
ADRB1(153)
Sequence:
MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGATPPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
89
Sheep
89
Pig
78
Dog
78
Rabbit
78
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08588 As Substrate

Site PTM Type Enzyme
Y157 Phosphorylation
S260 Phosphorylation
S274 Phosphorylation
S278 Phosphorylation
S312 Phosphorylation P17612 (PRKACA)
R357 Methylation
T404 Phosphorylation
S412 Phosphorylation
S423 Phosphorylation
S428 Phosphorylation
S461 Phosphorylation
S462 Phosphorylation
S475 Phosphorylation

Research Backgrounds

Function:

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling. Involved in the regulation of sleep/wake behaviors.

PTMs:

Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Early endosome.
Note: Colocalizes with RAPGEF2 at the plasma membrane (By similarity). Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via C-terminus PDZ motif) with RAPGEF2; the interaction is direct. Interacts with GOPC, MAGI3 and DLG4.

Family&Domains:

The PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

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