ADRA1A Antibody - #DF3506

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Product: ADRA1A Antibody
Catalog: DF3506
Description: Rabbit polyclonal antibody to ADRA1A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 51 KD; 51kD(Calculated).
Uniprot: P35348
RRID: AB_2835868

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(89%)
Clonality:
Polyclonal
Specificity:
ADRA1A Antibody detects endogenous levels of total ADRA1A.
RRID:
AB_2835868
Cite Format: Affinity Biosciences Cat# DF3506, RRID:AB_2835868.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADA1A_HUMAN; ADRA1A; Adra1c; ADRA1L1; Adrenergic alpha1A receptor; Adrenergic alpha1C receptor; Adrenergic receptor alpha 1a; Adrenergic receptor alpha 1c; Adrenergic, alpha 1A , receptor variant 1; Adrenergic, alpha 1A , receptor variant 11; Adrenergic, alpha 1A , receptor variant 3; Adrenergic, alpha 1A , receptor variant 5; Adrenergic, alpha 1A , receptor variant 8; Adrenoceptor alpha 1A; Alpha 1A adrenoreceptor; Alpha 1C adrenergic receptor; Alpha-1A adrenergic receptor; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; Alpha1A adrenergic receptor; ALPHA1AAR; G protein coupled receptor;

Immunogens

Immunogen:
Uniprot:

P35348(ADA1A_HUMAN) >>Visit HPA database.

Gene(ID):
ADRA1A(148)
Expression:
P35348 ADA1A_HUMAN:

Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

Sequence:
MVFLSGNASDSSNCTQPPAPVNISKAILLGVILGGLILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFEVLGYWAFGRVFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMALLCVWALSLVISIGPLFGWRQPAPEDETICQINEEPGYVLFSALGSFYLPLAIILVMYCRVYVVAKRESRGLKSGLKTDKSDSEQVTLRIHRKNAPAGGSGMASAKTKTHFSVRLLKFSREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPDFKPSETVFKIVFWLGYLNSCINPIIYPCSSQEFKKAFQNVLRIQCLCRKQSSKHALGYTLHPPSQAVEGQHKDMVRIPVGSRETFYRISKTDGVCEWKFFSSMPRGSARITVSKDQSSCTTARVRSKSFLQVCCCVGPSTPSLDKNHQVPTIKVHTISLSENGEEV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
89
Horse
78
Sheep
78
Bovine
75
Pig
67
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35348 As Substrate

Site PTM Type Enzyme
S220 Phosphorylation
S227 Phosphorylation
S229 Phosphorylation
T233 Phosphorylation
K239 Acetylation
K239 Ubiquitination
S246 Phosphorylation
S250 Phosphorylation
K252 Ubiquitination
K254 Ubiquitination
S258 Phosphorylation
T273 Phosphorylation
S304 Phosphorylation
S351 Phosphorylation
S352 Phosphorylation
S381 Phosphorylation
T384 Phosphorylation
S389 Phosphorylation
S401 Phosphorylation
S402 Phosphorylation
S407 Phosphorylation
T411 Phosphorylation
S413 Phosphorylation
T451 Phosphorylation

Research Backgrounds

Function:

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

PTMs:

C-terminal Ser or Thr residues may be phosphorylated.

Subcellular Location:

Nucleus membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cytoplasm. Membrane>Caveola.
Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.

Subunit Structure:

Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. Interacts with CAVIN4.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

Restrictive clause

 

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