5HT1F Receptor Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from mouse lung, using 5-HT-1F antibody.

FIGURE 3 Expression of 5-HT1F receptor in the spinal cord of sham and neuropathic pain rats.

FIGURE 4 Double immunofluorescence of 5-HT1F receptor and NeuN, GFAP, and Iba1 in the spinal cord of sham and neuropathic pain rats.

Product:	5HT1F Receptor Antibody
Catalog:	DF3499
Description:	Rabbit polyclonal antibody to 5HT1F Receptor
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	38 KD; 42kD(Calculated).
Uniprot:	P30939
RRID:	AB_2835861

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(82%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

5HT1F Receptor Antibody detects endogenous levels of total 5HT1F Receptor.

RRID:

AB_2835861
Cite Format: Affinity Biosciences Cat# DF3499, RRID:AB_2835861.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5 HT 1F; 5 HT1F; 5 hydroxytryptamine (serotonin) receptor 1F; 5 hydroxytryptamine 1F receptor; 5 hydroxytryptamine receptor 1E beta; 5 hydroxytryptamine receptor 1F; 5 hydroxytryptamine serotonin receptor 1E beta; 5 hydroxytryptamine serotonin receptor 1F; 5HT 1F; 5HT1F; HGNC5292; HTR 1F; HTR1EB; HTR1EL; HTR1F; MR 77; MR77; Serotonin receptor 1E beta; Serotonin receptor gene;

Immunogens

Immunogen:

Uniprot:

P30939(5HT1F_HUMAN) >>Visit HPA database.

Gene(ID):

HTR1F(3355)

Sequence:

P30939 5HT1F_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVIAAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVRESWIMGQVVCDIWLSVDITCCTCSILHLSAIALDRYRAITDAVEYARKRTPKHAGIMITIVWIISVFISMPPLFWRHQGTSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQASRIAKEEVNGQVLLESGEKSTKSVSTSYVLEKSLSDPSTDFDKIHSTVRSLRSEFKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNVCDKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Chicken

100

Rabbit

100

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30939 As Substrate

P30939

Site	PTM Type	Source
S27	Phosphorylation	Uniprot
T38	Phosphorylation	Uniprot
Y122	Phosphorylation	Uniprot
T126	Phosphorylation	Uniprot
Y131	Phosphorylation	Uniprot
Y184	Phosphorylation	Uniprot
Y201	Phosphorylation	Uniprot
Y202	Phosphorylation	Uniprot
Y205	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot

Research Backgrounds

P30939_5HT1F_HUMAN

Function:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

References

1). 5-HT1F Receptor Agonist Ameliorates Mechanical Allodynia in Neuropathic Pain via Induction of Mitochondrial Biogenesis and Suppression of Neuroinflammation. Frontiers in Pharmacology, 2022 (PubMed: 35308244) [IF=5.6]

Application: WB Species: Rat Sample:

FIGURE 3 Expression of 5-HT1F receptor in the spinal cord of sham and neuropathic pain rats. (A) The protein level of 5-HT1F receptor was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (** p < 0.01 compared with sham group, n = 6 in each group). (B) Histogram showing the mean intensity of 5-HT1F receptor immunofluorescent activity was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (****p < 0.0001 compared with sham group, n = 6 in each group). (C–H) Representative photomicrographs showed that neuropathic pain induced the downregulation of 5-HT1F receptor in the spinal cord.

Application: IF/ICC Species: Rat Sample:

FIGURE 4 Double immunofluorescence of 5-HT1F receptor and NeuN, GFAP, and Iba1 in the spinal cord of sham and neuropathic pain rats. (A) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of sham rats. (B) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of sham rats. (C) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of sham rats. (D) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of SNI rats. (E) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of SNI rats. (F) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of SNI rats. (G–I) Neuropathic pain induced decreased colocalization of 5-HT1F receptor with neurons (NeuN), while the colocalization of 5-HT1F receptor with astrocytes (GFAP), and microglia (Iba-1) remained sparse in the spinal cord (*p < 0.05 compared with sham group, n = 6 in each group).

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5HT1F Receptor Antibody - #DF3499