5HT1F Receptor Antibody - #DF3499

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Product: 5HT1F Receptor Antibody
Catalog: DF3499
Description: Rabbit polyclonal antibody to 5HT1F Receptor
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38 KD; 42kD(Calculated).
Uniprot: P30939
RRID: AB_2835861

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(82%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
5HT1F Receptor Antibody detects endogenous levels of total 5HT1F Receptor.
RRID:
AB_2835861
Cite Format: Affinity Biosciences Cat# DF3499, RRID:AB_2835861.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 HT 1F; 5 HT1F; 5 hydroxytryptamine (serotonin) receptor 1F; 5 hydroxytryptamine 1F receptor; 5 hydroxytryptamine receptor 1E beta; 5 hydroxytryptamine receptor 1F; 5 hydroxytryptamine serotonin receptor 1E beta; 5 hydroxytryptamine serotonin receptor 1F; 5HT 1F; 5HT1F; HGNC5292; HTR 1F; HTR1EB; HTR1EL; HTR1F; MR 77; MR77; Serotonin receptor 1E beta; Serotonin receptor gene;

Immunogens

Immunogen:
Uniprot:

P30939(5HT1F_HUMAN) >>Visit HPA database.

Gene(ID):
HTR1F(3355)
Sequence:
MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVIAAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVRESWIMGQVVCDIWLSVDITCCTCSILHLSAIALDRYRAITDAVEYARKRTPKHAGIMITIVWIISVFISMPPLFWRHQGTSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQASRIAKEEVNGQVLLESGEKSTKSVSTSYVLEKSLSDPSTDFDKIHSTVRSLRSEFKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNVCDKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30939 As Substrate

Site PTM Type Enzyme
S27 Phosphorylation
T38 Phosphorylation
Y122 Phosphorylation
T126 Phosphorylation
Y131 Phosphorylation
Y184 Phosphorylation
Y201 Phosphorylation
Y202 Phosphorylation
Y205 Phosphorylation
S233 Phosphorylation

Research Backgrounds

Function:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

References

1). 5-HT1F Receptor Agonist Ameliorates Mechanical Allodynia in Neuropathic Pain via Induction of Mitochondrial Biogenesis and Suppression of Neuroinflammation. Frontiers in Pharmacology (PubMed: 35308244) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 3 Expression of 5-HT1F receptor in the spinal cord of sham and neuropathic pain rats. (A) The protein level of 5-HT1F receptor was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (** p < 0.01 compared with sham group, n = 6 in each group). (B) Histogram showing the mean intensity of 5-HT1F receptor immunofluorescent activity was significantly decreased in SNI rats, beginning at day 3 after surgery and remain at low level until day 14 (****p < 0.0001 compared with sham group, n = 6 in each group). (C–H) Representative photomicrographs showed that neuropathic pain induced the downregulation of 5-HT1F receptor in the spinal cord.

Application: IF/ICC    Species: Rat    Sample:

FIGURE 4 Double immunofluorescence of 5-HT1F receptor and NeuN, GFAP, and Iba1 in the spinal cord of sham and neuropathic pain rats. (A) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of sham rats. (B) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of sham rats. (C) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of sham rats. (D) Double immunofluorescence of 5-HT1F receptor and NeuN in the spinal cord of SNI rats. (E) Double immunofluorescence of 5-HT1F receptor and Iba-1 in the spinal cord of SNI rats. (F) Double immunofluorescence of 5-HT1F receptor and GFAP in the spinal cord of SNI rats. (G–I) Neuropathic pain induced decreased colocalization of 5-HT1F receptor with neurons (NeuN), while the colocalization of 5-HT1F receptor with astrocytes (GFAP), and microglia (Iba-1) remained sparse in the spinal cord (*p < 0.05 compared with sham group, n = 6 in each group).

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