THRB Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/4

AF0357 at 1/100 staining human liver tissue sections by IHC-P.

AF0357 staining A549 cells by IF/ICC.

Fig.

Figure 5 Immune Infiltration analyse of TICs and identification of prognostic target gene in UCEC.

Product:	THRB Antibody
Catalog:	AF0357
Description:	Rabbit polyclonal antibody to THRB
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.:	70kDa; 70kD(Calculated).
Uniprot:	P00734
RRID:	AB_2833522

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(82%), Bovine(82%), Horse(82%), Sheep(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

THRB Antibody detects endogenous levels of total THRB.

RRID:

AB_2833522
Cite Format: Affinity Biosciences Cat# AF0357, RRID:AB_2833522.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

coagulation factor II (thrombin); Coagulation factor II; F2; Factor II; Prepro coagulation factor II; Prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; THRB; THRB_HUMAN; Thrombin heavy chain;

Immunogens

Immunogen:

Uniprot:

P00734(THRB_HUMAN) >>Visit HPA database.

Gene(ID):

F2(2147)

Expression:

P00734 THRB_HUMAN:

Expressed by the liver and secreted in plasma.

Description:

prothrombin Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Belongs to the peptidase S1 family.

Sequence:

P00734 THRB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

Horse

Bovine

Sheep

Zebrafish

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00734 As Substrate

P00734

Site	PTM Type	Source
N121	N-Glycosylation	Uniprot
N143	N-Glycosylation	Uniprot
S199	Phosphorylation	Uniprot
Y320	Phosphorylation	Uniprot
S331	Phosphorylation	Uniprot
N416	N-Glycosylation	Uniprot
Y434	Phosphorylation	Uniprot
S539	Phosphorylation	Uniprot
T540	Phosphorylation	Uniprot
K599	Acetylation	Uniprot

Research Backgrounds

P00734_THRB_HUMAN

Function:

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

PTMs:

The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.

N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

Subcellular Location:

Secreted>Extracellular space.

Tissue Specificity:

Expressed by the liver and secreted in plasma.

Subunit Structure:

Heterodimer (named alpha-thrombin) of a light and a heavy chain; disulfide-linked. Forms a heterodimer with SERPINA5.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Development of a necroptosis-related prognostic model for uterine corpus endometrial carcinoma. Scientific reports, 2024 (PubMed: 38383747) [IF=4.6]

Application: WB Species: Human Sample: tumor tissues

Figure 5 Immune Infiltration analyse of TICs and identification of prognostic target gene in UCEC. (A) Bar chart depicted the composition of immune cells in each sample from the TCGA-UCEC cohort. (B) Violin plot illustrated the differential infiltration of 22 types of TICs. (C) Venn diagram presented 11 immune-related target genes. (D) Survival analysis demonstrated a significant impact of the THRB gene on prognosis (P

2). KMT2D Regulates the NCOA6/THRB Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer. Frontiers in Bioscience - Elite, 2023 (PubMed: 36722273)

Application: WB Species: Human Sample: thyroid papillary carcinoma cells

Fig. 3. The mechanism of KMT2D on papillary thyroid carcinoma cells. (a) ChIP assay. (b) qRT-PCR analysis. (c) Western blotting analysis. A, si-NC; B, si-KMT2D (1); C, si- KMT2D (1).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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THRB Antibody - #AF0357