14-3-3 eta Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat testis, using 14-3-3 eta Antibody.

Product:	14-3-3 eta Antibody
Catalog:	DF3492
Description:	Rabbit polyclonal antibody to 14-3-3 eta
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	42 KD; 28kD(Calculated).
Uniprot:	Q04917
RRID:	AB_2835854

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

14-3-3 eta Antibody detects endogenous levels of total 14-3-3 eta.

RRID:

AB_2835854
Cite Format: Affinity Biosciences Cat# DF3492, RRID:AB_2835854.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

14 3 3 protein eta; 14-3-3 eta; 14-3-3 protein eta; 1433F_HUMAN; Brain protein 14-3-3, eta isoform; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, eta isoform; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1; YWHA1; Ywhah;

Immunogens

Immunogen:

Uniprot:

Q04917(1433F_HUMAN) >>Visit HPA database.

Gene(ID):

YWHAH(7533)

Expression:

Q04917 1433F_HUMAN:

Expressed mainly in the brain and present in other tissues albeit at lower levels.

Sequence:

Q04917 1433F_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04917 As Substrate

Q04917

Site	PTM Type	Enzyme	Source
Y20	Phosphorylation		Uniprot
S25	Phosphorylation		Uniprot
S38	Phosphorylation		Uniprot
R42	Methylation		Uniprot
S46	Phosphorylation		Uniprot
Y49	Phosphorylation		Uniprot
K50	Acetylation		Uniprot
K50	Methylation		Uniprot
K50	Ubiquitination		Uniprot
S58	Phosphorylation		Uniprot
S59	Phosphorylation	Q05655 (PRKCD)	Uniprot
S65	Phosphorylation		Uniprot
K69	Acetylation		Uniprot
K69	Ubiquitination		Uniprot
K77	Ubiquitination		Uniprot
K91	Ubiquitination		Uniprot
K106	Ubiquitination		Uniprot
K110	Ubiquitination		Uniprot
S119	Phosphorylation		Uniprot
K120	Acetylation		Uniprot
K120	Ubiquitination		Uniprot
Y123	Phosphorylation		Uniprot
K125	Acetylation		Uniprot
K125	Ubiquitination		Uniprot
K127	Ubiquitination		Uniprot
Y130	Phosphorylation		Uniprot
Y131	Phosphorylation		Uniprot
S139	Phosphorylation		Uniprot
K142	Acetylation		Uniprot
K142	Ubiquitination		Uniprot
K143	Ubiquitination		Uniprot
S145	Phosphorylation		Uniprot
Y154	Phosphorylation		Uniprot
K155	Ubiquitination		Uniprot
T168	Phosphorylation		Uniprot
S215	Phosphorylation		Uniprot
Y216	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
T220	Phosphorylation		Uniprot

Research Backgrounds

Q04917_1433F_HUMAN

Function:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

PTMs:

Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.

Tissue Specificity:

Expressed mainly in the brain and present in other tissues albeit at lower levels.

Subunit Structure:

Homodimer (By similarity). Interacts with many nuclear hormone receptors and cofactors including AR, ESR1, ESR2, MC2R, NR3C1, NRIP1, PPARBP and THRA. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with ARHGEF28 and CDK16 (By similarity). Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with KCNK18 in a phosphorylation-dependent manner. Interacts with SAMSN1 (By similarity). Interacts with the 'Ser-241' phosphorylated form of PDPK1. Interacts with the 'Thr-369' phosphorylated form of DAPK2. Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with SLITRK1.

Family&Domains:

Belongs to the 14-3-3 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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For Research Use Only.
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14-3-3 eta Antibody - #DF3492