Product: CHKB Antibody
Catalog: DF3477
Description: Rabbit polyclonal antibody to CHKB
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 45 KD; 45kD(Calculated).
Uniprot: Q9Y259
RRID: AB_2835840

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CHKB Antibody detects endogenous levels of total CHKB.
RRID:
AB_2835840
Cite Format: Affinity Biosciences Cat# DF3477, RRID:AB_2835840.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CHETK; Chetk-beta; Chkb; Chkb choline kinase beta; Chkb protein; CHKB_HUMAN; CHKL; Choline kinase beta; Choline kinase-like; Choline kinase-like protein; Choline/ethanolamine kinase; choline/ethanolamine kinase beta; Choline/ethanolamine kinase, beta; CK; CK/EK; CK/EK-beta; CKB; CKEKB; EK; EKB; Ethanolamine kinase; Ethanolamine kinase beta; Ethanolamine kinase, beta; MDCMC;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAEATAVAGSGAVGGCLAKDGLQQSKCPDTTPKRRRASSLSRDAERRAYQWCREYLGGAWRRVQPEELRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAERSLGPQLYGVFPEGRLEQYIPSRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHWLFGTMERYLKQIQDLPPTGLPEMNLLEMYSLKDEMGNLRKLLESTPSPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQLTSVHSSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Dog
91
Rabbit
82
Horse
73
Sheep
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y259 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S39 Phosphorylation
S40 Phosphorylation
S42 Phosphorylation
Y72 Phosphorylation
S75 Phosphorylation
S79 Phosphorylation
K321 Ubiquitination
Y381 Phosphorylation
S394 Phosphorylation

Research Backgrounds

Function:

Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.

Subunit Structure:

Homodimer, and heterodimer with CHKA.

Family&Domains:

Belongs to the choline/ethanolamine kinase family.

Research Fields

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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