ZNF148 Antibody - #DF3476

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Product: ZNF148 Antibody
Catalog: DF3476
Description: Rabbit polyclonal antibody to ZNF148
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 89 KD; 89kD(Calculated).
Uniprot: Q9UQR1
RRID: AB_2835839

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ZNF148 Antibody detects endogenous levels of total ZNF148.
RRID:
AB_2835839
Cite Format: Affinity Biosciences Cat# DF3476, RRID:AB_2835839.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148;

Immunogens

Immunogen:
Uniprot:

Q9UQR1(ZN148_HUMAN) >>Visit HPA database.

Gene(ID):
ZNF148(7707)
Sequence:
MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQKYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHENHDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPLYSSSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEESKVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALNVGTIASQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISFSVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFPLVNVNDNRAGMTSSPDATTGQTFG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
67
Pig
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQR1 As Substrate

Site PTM Type Enzyme
T25 Phosphorylation
T37 Phosphorylation
S51 Phosphorylation
K115 Sumoylation
K132 Sumoylation
S150 Phosphorylation
K153 Ubiquitination
K166 Ubiquitination
T167 Phosphorylation
T194 Phosphorylation
K197 Ubiquitination
S202 Phosphorylation Q13315 (ATM)
T222 Phosphorylation
K239 Sumoylation
K239 Ubiquitination
S250 Phosphorylation
Y259 Phosphorylation
K291 Methylation
K291 Ubiquitination
T296 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
S304 Phosphorylation
T305 Phosphorylation
S306 Phosphorylation
S311 Phosphorylation
S325 Phosphorylation
S331 Phosphorylation
S336 Phosphorylation
K342 Ubiquitination
Y345 Phosphorylation
Y349 Phosphorylation
K356 Sumoylation
S400 Phosphorylation
K402 Ubiquitination
T410 Phosphorylation
S412 Phosphorylation
S415 Phosphorylation
Y417 Phosphorylation
K421 Ubiquitination
S423 Phosphorylation
K424 Sumoylation
K424 Ubiquitination
Y425 Phosphorylation
K432 Ubiquitination
S438 Phosphorylation
K456 Acetylation
K456 Ubiquitination
K472 Ubiquitination
K533 Ubiquitination
K607 Acetylation
K607 Ubiquitination
S627 Phosphorylation
S661 Phosphorylation
S665 Phosphorylation
T669 Phosphorylation
T670 Phosphorylation
R734 Methylation
S740 Phosphorylation
S749 Phosphorylation
S784 Phosphorylation

Research Backgrounds

Function:

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

PTMs:

Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with HNRNPDL. Interacts with the 5FMC complex; the interaction requires association with CHTOP. Interacts with CAVIN1 (By similarity).

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

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