Mlx Antibody - #DF3466

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Product: Mlx Antibody
Catalog: DF3466
Description: Rabbit polyclonal antibody to Mlx
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33 KD; 33kD(Calculated).
Uniprot: Q9UH92
RRID: AB_2835830

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
Mlx Antibody detects endogenous levels of total Mlx.
RRID:
AB_2835830
Cite Format: Affinity Biosciences Cat# DF3466, RRID:AB_2835830.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHd13; BigMax protein; Class D basic helix-loop-helix protein 13; MAD7; Max like bHLHZip protein; Max like protein X; Max-like bHLHZip protein; Max-like protein X; MLX; MLX_HUMAN; MLXIP; MONDOA; MXD7; Protein BigMax; Protein Mlx; TCFL4; transcription factor like 4; Transcription factor like protein 4; Transcription factor-like protein 4;

Immunogens

Immunogen:
Uniprot:

Q9UH92(MLX_HUMAN) >>Visit HPA database.

Gene(ID):
MLX(6945)
Expression:
Q9UH92 MLX_HUMAN:

Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.

Sequence:
MTEPGASPEDPWVKASPVGAHAGEGRAGRARARRGAGRRGASLLSPKSPTLSVPRGCREDSSHPACAKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
91
Chicken
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UH92 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
R26 Methylation
R29 Methylation
R31 Methylation
S42 Phosphorylation
S45 Phosphorylation
S48 Phosphorylation
T50 Phosphorylation
S74 Phosphorylation
S77 Phosphorylation
T87 Phosphorylation
S91 Phosphorylation
S94 Phosphorylation
S98 Phosphorylation
T102 Phosphorylation
S105 Phosphorylation
S106 Phosphorylation
T110 Phosphorylation
S115 Phosphorylation
K140 Ubiquitination
K170 Ubiquitination
K173 Ubiquitination
K179 Ubiquitination
K189 Ubiquitination
K193 Ubiquitination
T206 Phosphorylation
K209 Ubiquitination
K212 Ubiquitination
K220 Ubiquitination

Research Backgrounds

Function:

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

Subcellular Location:

Cytoplasm.
Note: Found predominantly in the cytoplasm (PubMed:10918583).

Cytoplasm.
Note: Found predominantly in the cytoplasm (PubMed:10918583).

Nucleus.
Note: Found predominantly in the nucleus (PubMed:10918583).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

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