DBF4 Antibody - #DF3462

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Product: DBF4 Antibody
Catalog: DF3462
Description: Rabbit polyclonal antibody to DBF4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Dog
Mol.Wt.: 77 KD; 77kD(Calculated).
Uniprot: Q9UBU7
RRID: AB_2835826

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(86%), Horse(83%), Dog(86%)
Clonality:
Polyclonal
Specificity:
DBF4 Antibody detects endogenous levels of total DBF4.
RRID:
AB_2835826
Cite Format: Affinity Biosciences Cat# DF3462, RRID:AB_2835826.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4 homolog; DBF4 type zinc finger containing protein 1; DBF4 zinc finger A; DBF4 zinc finger; DBF4, S. cerevisiae, homolog of; DBF4-type zinc finger-containing protein 1; DBF4A; DBF4A_HUMAN; Dumbbell former 4, S. cerevisiae, homolog of; OTTHUMP00000161661; Protein DBF4 homolog A; ZDBF1; Zinc finger, DBF-type containing 1;

Immunogens

Immunogen:
Uniprot:

Q9UBU7(DBF4A_HUMAN) >>Visit HPA database.

Gene(ID):
DBF4(10926)
Expression:
Q9UBU7 DBF4A_HUMAN:

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Sequence:
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Dog
86
Horse
83
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBU7 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
K21 Sumoylation
K81 Ubiquitination
T98 Phosphorylation
S103 Phosphorylation
S107 Phosphorylation
S130 Phosphorylation
K140 Ubiquitination
Y177 Phosphorylation
T194 Phosphorylation
S195 Phosphorylation
K201 Ubiquitination
S260 Phosphorylation
T273 Phosphorylation
Y278 Phosphorylation
T281 Phosphorylation
S282 Phosphorylation
Y303 Phosphorylation
S312 Phosphorylation
T345 Phosphorylation
Y353 Phosphorylation
S354 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S362 Phosphorylation
S364 Phosphorylation
S381 Phosphorylation
K393 Ubiquitination
K408 Sumoylation
S413 Phosphorylation
S420 Phosphorylation
K429 Ubiquitination
S435 Phosphorylation
T449 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
K455 Ubiquitination
S465 Phosphorylation
T468 Phosphorylation
S470 Phosphorylation
S502 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
S508 Phosphorylation
T510 Phosphorylation
T535 Phosphorylation
S539 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
T553 Phosphorylation
S568 Phosphorylation
S571 Phosphorylation
K579 Sumoylation
S612 Phosphorylation
S613 Phosphorylation
S629 Phosphorylation
T635 Phosphorylation
T638 Phosphorylation
S664 Phosphorylation
S665 Phosphorylation

Research Backgrounds

Function:

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

PTMs:

Phosphorylation increases its interaction with PSIP1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Subunit Structure:

Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. Interacts (via IBM motifs) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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