DUS2L Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	DUS2L Antibody
Catalog:	DF3452
Description:	Rabbit polyclonal antibody to DUS2L
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	55 KD; 55kD(Calculated).
Uniprot:	Q9NX74
RRID:	AB_2835818

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Bovine(86%), Horse(88%), Sheep(86%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

DUS2L Antibody detects endogenous levels of total DUS2L.

RRID:

AB_2835818
Cite Format: Affinity Biosciences Cat# DF3452, RRID:AB_2835818.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310016K04Rik; dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae); DUS2; FLJ20399; SMM1; tRNA dihydrouridine synthase 2 like; Up regulated in lung cancer; URLC8;

Immunogens

Immunogen:

Uniprot:

Q9NX74(DUS2L_HUMAN) >>Visit HPA database.

Gene(ID):

DUS2(54920)

Expression:

Q9NX74 DUS2L_HUMAN:

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).

Sequence:

Q9NX74 DUS2L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NX74 As Substrate

Q9NX74

Site	PTM Type	Source
S5	Phosphorylation	Uniprot
Y10	Phosphorylation	Uniprot
T24	Phosphorylation	Uniprot
K118	Ubiquitination	Uniprot
S121	Phosphorylation	Uniprot
K123	Ubiquitination	Uniprot
K146	Ubiquitination	Uniprot
K155	Ubiquitination	Uniprot
K170	Ubiquitination	Uniprot
T175	Phosphorylation	Uniprot
T234	Phosphorylation	Uniprot
K266	Ubiquitination	Uniprot
Y274	Phosphorylation	Uniprot
Y283	Phosphorylation	Uniprot
K337	Ubiquitination	Uniprot
K417	Ubiquitination	Uniprot
S445	Phosphorylation	Uniprot
S447	Phosphorylation	Uniprot
K477	Ubiquitination	Uniprot
S484	Phosphorylation	Uniprot
S488	Phosphorylation	Uniprot

Research Backgrounds

Q9NX74_DUS2L_HUMAN

Function:

Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum.
Note: Mainly at the endoplasmic reticulum.

Tissue Specificity:

Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level).

Subunit Structure:

Interacts with EPRS1. Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).

Family&Domains:

Efficient dihydrouridine synthesis requires the presence of both the catalytic domain and the C-terminal RNA-binding DRBM domain.

Belongs to the Dus family. Dus2 subfamily.

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DUS2L Antibody - #DF3452