MED17 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 cells, using MED17 antibody.

Product:	MED17 Antibody
Catalog:	DF3449
Description:	Rabbit polyclonal antibody to MED17
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	73 KD; 73kD(Calculated).
Uniprot:	Q9NVC6
RRID:	AB_2835815

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

MED17 Antibody detects endogenous levels of total MED17.

RRID:

AB_2835815
Cite Format: Affinity Biosciences Cat# DF3449, RRID:AB_2835815.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

77-KD subunit; Activator recruited cofactor 77 kDa component; Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; CRSP6; CRSP77; DRIP80; med17; MED17_HUMAN; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of; Thyroid hormone receptor-associated protein complex 80 kDa component; Thyroid hormone receptor-associated protein, 80-KD subunit; Transcriptional coactivator CRSP77; Trap80; VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD; Vitamin D3 receptor-interacting protein complex 80 kDa component;

Immunogens

Immunogen:

Uniprot:

Q9NVC6(MED17_HUMAN) >>Visit HPA database.

Gene(ID):

MED17(9440)

Expression:

Q9NVC6 MED17_HUMAN:

Ubiquitous.

Sequence:

Q9NVC6 MED17_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVC6 As Substrate

Q9NVC6

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
K17	Ubiquitination	Uniprot
S37	Phosphorylation	Uniprot
S50	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
S55	Phosphorylation	Uniprot
K123	Ubiquitination	Uniprot
T143	Phosphorylation	Uniprot
K149	Ubiquitination	Uniprot
K162	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot
S185	Phosphorylation	Uniprot
K202	Ubiquitination	Uniprot
K226	Ubiquitination	Uniprot
K234	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
Y256	Phosphorylation	Uniprot
K263	Ubiquitination	Uniprot
K278	Acetylation	Uniprot
K278	Ubiquitination	Uniprot
S284	Phosphorylation	Uniprot
K285	Ubiquitination	Uniprot
S288	Phosphorylation	Uniprot
K294	Ubiquitination	Uniprot
K305	Ubiquitination	Uniprot
K319	Ubiquitination	Uniprot
K353	Acetylation	Uniprot
K356	Acetylation	Uniprot
K356	Ubiquitination	Uniprot
K361	Acetylation	Uniprot
K361	Ubiquitination	Uniprot
K403	Ubiquitination	Uniprot
S408	Phosphorylation	Uniprot
K415	Ubiquitination	Uniprot
K430	Ubiquitination	Uniprot
K436	Ubiquitination	Uniprot
K591	Ubiquitination	Uniprot
K606	Ubiquitination	Uniprot
K614	Ubiquitination	Uniprot
K623	Ubiquitination	Uniprot
K629	Ubiquitination	Uniprot
Y637	Phosphorylation	Uniprot
S647	Phosphorylation	Uniprot

Research Backgrounds

Q9NVC6_MED17_HUMAN

Function:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with GATA1 and PPARG (By similarity). Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2.

Family&Domains:

Belongs to the Mediator complex subunit 17 family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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MED17 Antibody - #DF3449