IARS2 Antibody - #DF3448

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Product: IARS2 Antibody
Catalog: DF3448
Description: Rabbit polyclonal antibody to IARS2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 114 KD; 114kD(Calculated).
Uniprot: Q9NSE4
RRID: AB_2835814

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(90%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IARS2 Antibody detects endogenous levels of total IARS2.
RRID:
AB_2835814
Cite Format: Affinity Biosciences Cat# DF3448, RRID:AB_2835814.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2010002H18Rik; C79125; EC 6.1.1.5; FLJ10326; Iars2; IleRS; isoleucine tRNA ligase 2, mitochondrial; isoleucine tRNA ligase, mitochondrial; isoleucine tRNA synthetase 2, mitochondrial; Isoleucine--tRNA ligase; isoleucine-tRNA synthetase 2; isoleucyl tRNA synthetase 2, mitochondrial; Isoleucyl-tRNA synthetase; MGC63429; mitochondrial; mitochondrial isoleucine tRNA synthetase; rCG_20084; RGD1311857; SYIM_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9NSE4(SYIM_HUMAN) >>Visit HPA database.

Gene(ID):
IARS2(55699)
Sequence:
MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
90
Bovine
90
Chicken
78
Zebrafish
75
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NSE4 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
S46 Phosphorylation
S57 Phosphorylation
Y60 Phosphorylation
K133 Ubiquitination
S186 Phosphorylation
K189 Acetylation
K189 Ubiquitination
K222 Acetylation
R225 Methylation
Y231 Phosphorylation
K233 Acetylation
Y237 Phosphorylation
K241 Acetylation
K241 Methylation
K241 Ubiquitination
T251 Phosphorylation
Y260 Phosphorylation
K445 Ubiquitination
K455 Sumoylation
K458 Ubiquitination
K479 Acetylation
K493 Ubiquitination
K500 Acetylation
K540 Ubiquitination
Y543 Phosphorylation
S547 Phosphorylation
Y621 Phosphorylation
T657 Phosphorylation
K687 Ubiquitination
K725 Acetylation
K775 Acetylation
K781 Acetylation
Y798 Phosphorylation
K803 Acetylation
K872 Ubiquitination
S881 Phosphorylation
S888 Phosphorylation
K896 Ubiquitination
T950 Phosphorylation
T982 Phosphorylation
C1005 S-Nitrosylation

Research Backgrounds

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the class-I aminoacyl-tRNA synthetase family.

Research Fields

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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For Research Use Only.
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