DCP1A Antibody - #DF3443

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Product: DCP1A Antibody
Catalog: DF3443
Description: Rabbit polyclonal antibody to DCP1A
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 75 KD; 63kD(Calculated).
Uniprot: Q9NPI6
RRID: AB_2835809

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DCP1A Antibody detects endogenous levels of total DCP1A.
RRID:
AB_2835809
Cite Format: Affinity Biosciences Cat# DF3443, RRID:AB_2835809.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCP1 decapping enzyme homolog A; Dcp1a; DCP1A_HUMAN; Decapping enzyme hDcp1a; Decapping mRNA 1A; HSA275986; mRNA decapping enzyme 1A; mRNA-decapping enzyme 1A; Nbla00360; Putative protein product of Nbla00360; Smad4 interacting transcriptional co activator; Smad4-interacting transcriptional co-activator; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; Transcription factor SMIF;

Immunogens

Immunogen:
Uniprot:

Q9NPI6(DCP1A_HUMAN) >>Visit HPA database.

Gene(ID):
DCP1A(55802)
Expression:
Q9NPI6 DCP1A_HUMAN:

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

Sequence:
MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPRQRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPI6 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
K47 Ubiquitination
S60 Phosphorylation
S62 Phosphorylation
Y64 Phosphorylation
K120 Ubiquitination
K140 Ubiquitination
S142 Phosphorylation
S144 Phosphorylation
S176 Phosphorylation
S179 Phosphorylation
S180 Phosphorylation
S186 Phosphorylation
T195 Phosphorylation
T197 Phosphorylation
K215 Ubiquitination
S226 Phosphorylation
K229 Ubiquitination
T290 Phosphorylation
T295 Phosphorylation
T309 Phosphorylation
Y310 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation P45983 (MAPK8)
S319 Phosphorylation
T321 Phosphorylation
S334 Phosphorylation
T340 Phosphorylation
K346 Ubiquitination
T347 Phosphorylation
T348 Phosphorylation
S353 Phosphorylation
S373 Phosphorylation
R376 Methylation
K397 Ubiquitination
T401 Phosphorylation
S422 Phosphorylation
T430 Phosphorylation
S433 Phosphorylation
K520 Ubiquitination
S522 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
T528 Phosphorylation
T531 Phosphorylation
S534 Phosphorylation
K537 Ubiquitination
S539 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation

Research Backgrounds

Function:

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

Subcellular Location:

Cytoplasm>P-body. Nucleus.
Note: Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB) (PubMed:16364915). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (PubMed:11836524).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.

Subunit Structure:

Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with DCP1B. Interacts with DCP2. Interacts with DDX17 in an RNA-independent manner. Interacts with PNRC2. Interacts with SMAD4. Interacts with UPF1. Interacts with ZC3HAV1. Interacts with ZFP36L1. Interacts with NBDY.

Family&Domains:

Belongs to the DCP1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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