Product: UTP14A Antibody
Catalog: DF3418
Description: Rabbit polyclonal antibody to UTP14A
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Dog
Mol.Wt.: 85 KD; 88kD(Calculated).
Uniprot: Q9BVJ6
RRID: AB_2835800

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Horse(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
UTP14A Antibody detects endogenous levels of total UTP14A.
RRID:
AB_2835800
Cite Format: Affinity Biosciences Cat# DF3418, RRID:AB_2835800.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen NY CO 16; KIAA0266; SDCCAG16; Serologically defined colon cancer antigen 16; U3 small nucleolar RNA associated protein 14 homolog A; UTP14;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BVJ6 UT14A_HUMAN:

Ubiquitously expressed.

Sequence:
MTANRLAESLLALSQQEELADLPKDYLLSESEDEGDNDGERKHQKLLEAISSLDGKNRRKLAERSEASLKVSEFNVSSEGSGEKLVLADLLEPVKTSSSLATVKKQLSRVKSKKTVELPLNKEEIERIHREVAFNKTAQVLSKWDPVVLKNRQAEQLVFPLEKEEPAIAPIEHVLSGWKARTPLEQEIFNLLHKNKQPVTDPLLTPVEKASLRAMSLEEAKMRRAELQRARALQSYYEAKARREKKIKSKKYHKVVKKGKAKKALKEFEQLRKVNPAAALEELEKIEKARMMERMSLKHQNSGKWAKSKAIMAKYDLEARQAMQEQLSKNKELTQKLQVASESEEEEGGTEDVEELLVPDVVNEVQMNADGPNPWMLRSCTSDTKEAATQEDPEQLPELEAHGVSESEGEERPVAEEEILLREFEERRSLRKRSELSQDAEPAGSQETKDSGSQEVLSELRVLSQKLKENHQSRKQKASSEGTIPQVQREEPAPEEEEPLLLQRPERVQTLEELEELGKEECFQNKELPRPVLEGQQSERTPNNRPDAPKEKKKKEQMIDLQNLLTTQSPSVKSLAVPTIEELEDEEERNHRQMIKEAFAGDDVIRDFLKEKREAVEASKPKDVDLTLPGWGEWGGVGLKPSAKKRRRFLIKAPEGPPRKDKNLPNVIINEKRNIHAAAHQVRVLPYPFTHHWQFERTIQTPIGSTWNTQRAFQKLTTPKVVTKPGHIINPIKAEDVGYRSSSRSDLSVIQRNPKRITTRHKKQLKKCSVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Dog
88
Bovine
75
Rabbit
75
Chicken
67
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BVJ6 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S14 Phosphorylation
Y26 Phosphorylation
S29 Phosphorylation
S31 Phosphorylation
K45 Sumoylation
S52 Phosphorylation
K56 Acetylation
S65 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
S81 Phosphorylation
S99 Phosphorylation
T102 Phosphorylation
K136 Methylation
K143 Ubiquitination
K179 Ubiquitination
K194 Ubiquitination
K196 Ubiquitination
T205 Phosphorylation
K288 Ubiquitination
K298 Methylation
K307 Methylation
K309 Methylation
K314 Sumoylation
S341 Phosphorylation
S343 Phosphorylation
T389 Phosphorylation
S405 Phosphorylation
S407 Phosphorylation
S429 Phosphorylation
S434 Phosphorylation
S437 Phosphorylation
S445 Phosphorylation
T448 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation
S458 Phosphorylation
S479 Phosphorylation
S480 Phosphorylation
T510 Phosphorylation
S538 Phosphorylation
T541 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
K620 Sumoylation
K640 Ubiquitination
S642 Phosphorylation
K652 Methylation
K662 Ubiquitination
K672 Ubiquitination
T701 Phosphorylation
K733 Acetylation
K733 Sumoylation
S741 Phosphorylation
S743 Phosphorylation
S748 Phosphorylation

Research Backgrounds

Function:

May be required for ribosome biogenesis.

PTMs:

Citrullinated by PADI4.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Family&Domains:

Belongs to the UTP14 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). Overexpression of U three protein 14a (UTP14a) is associated with poor prognosis of esophageal squamous cell carcinoma. Thoracic Cancer, 2019 (PubMed: 31496055) [IF=2.9]

Application: IHC    Species: human    Sample: esophageal squamous cell carcinoma (ESCC) and CDM tissues

Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.

Application: WB    Species: human    Sample: ESCC and CDM tissue

Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.(e–f) Negative-to-moderate UTP14a expression in CDM tissues. (h) Western blot analysis of six pairs of ESCC and CDM tissue.

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