UTP14A Antibody - #DF3418
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| Product: | UTP14A Antibody |
| Catalog: | DF3418 |
| Description: | Rabbit polyclonal antibody to UTP14A |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Dog |
| Mol.Wt.: | 85 KD; 88kD(Calculated). |
| Uniprot: | Q9BVJ6 |
| RRID: | AB_2835800 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(88%), Horse(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
UTP14A Antibody detects endogenous levels of total UTP14A.
RRID:
AB_2835800
Cite Format: Affinity Biosciences Cat# DF3418, RRID:AB_2835800.
Cite Format: Affinity Biosciences Cat# DF3418, RRID:AB_2835800.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Antigen NY CO 16; KIAA0266; SDCCAG16; Serologically defined colon cancer antigen 16; U3 small nucleolar RNA associated protein 14 homolog A; UTP14;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q9BVJ6 UT14A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTANRLAESLLALSQQEELADLPKDYLLSESEDEGDNDGERKHQKLLEAISSLDGKNRRKLAERSEASLKVSEFNVSSEGSGEKLVLADLLEPVKTSSSLATVKKQLSRVKSKKTVELPLNKEEIERIHREVAFNKTAQVLSKWDPVVLKNRQAEQLVFPLEKEEPAIAPIEHVLSGWKARTPLEQEIFNLLHKNKQPVTDPLLTPVEKASLRAMSLEEAKMRRAELQRARALQSYYEAKARREKKIKSKKYHKVVKKGKAKKALKEFEQLRKVNPAAALEELEKIEKARMMERMSLKHQNSGKWAKSKAIMAKYDLEARQAMQEQLSKNKELTQKLQVASESEEEEGGTEDVEELLVPDVVNEVQMNADGPNPWMLRSCTSDTKEAATQEDPEQLPELEAHGVSESEGEERPVAEEEILLREFEERRSLRKRSELSQDAEPAGSQETKDSGSQEVLSELRVLSQKLKENHQSRKQKASSEGTIPQVQREEPAPEEEEPLLLQRPERVQTLEELEELGKEECFQNKELPRPVLEGQQSERTPNNRPDAPKEKKKKEQMIDLQNLLTTQSPSVKSLAVPTIEELEDEEERNHRQMIKEAFAGDDVIRDFLKEKREAVEASKPKDVDLTLPGWGEWGGVGLKPSAKKRRRFLIKAPEGPPRKDKNLPNVIINEKRNIHAAAHQVRVLPYPFTHHWQFERTIQTPIGSTWNTQRAFQKLTTPKVVTKPGHIINPIKAEDVGYRSSSRSDLSVIQRNPKRITTRHKKQLKKCSVD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
88
Horse
88
Dog
88
Bovine
75
Rabbit
75
Chicken
67
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BVJ6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K45 | Sumoylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| K56 | Acetylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| K136 | Methylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K298 | Methylation | Uniprot | |
| K307 | Methylation | Uniprot | |
| K309 | Methylation | Uniprot | |
| K314 | Sumoylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| T541 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| K620 | Sumoylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| K652 | Methylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| T701 | Phosphorylation | Uniprot | |
| K733 | Acetylation | Uniprot | |
| K733 | Sumoylation | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
May be required for ribosome biogenesis.
PTMs:
Citrullinated by PADI4.
Subcellular Location:
Nucleus>Nucleolus.
Tissue Specificity:
Ubiquitously expressed.
Family&Domains:
Belongs to the UTP14 family.
Research Fields
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
References
1). Overexpression of U three protein 14a (UTP14a) is associated with poor prognosis of esophageal squamous cell carcinoma. Thoracic Cancer, 2019
(PubMed: 31496055)
[IF=2.9]
Application: IHC Species: human Sample: esophageal squamous cell carcinoma (ESCC) and CDM tissues
Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.
Application: WB Species: human Sample: ESCC and CDM tissue
Figure 1 |IHC staining and Western blot analysis of UTP14a in esophageal squamous cell carcinoma (ESCC) and CDM tissues. (a–d) Negative to strong UTP14a expression in ESCC tissues.(e–f) Negative-to-moderate UTP14a expression in CDM tissues. (h) Western blot analysis of six pairs of ESCC and CDM tissue.
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