IP3KC Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HT-29 cells, using IP3KC antibody.

Product:	IP3KC Antibody
Catalog:	DF3397
Description:	Rabbit polyclonal antibody to IP3KC
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	102 KD; 75kD(Calculated).
Uniprot:	Q96DU7
RRID:	AB_2835779

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(82%)

Clonality:

Polyclonal

Specificity:

IP3KC Antibody detects endogenous levels of total IP3KC.

RRID:

AB_2835779
Cite Format: Affinity Biosciences Cat# DF3397, RRID:AB_2835779.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4; 5-trisphosphate 3-kinase C; Inositol 1; inositol 1,4,5 trisphosphate 3 kinase C; Inositol trisphosphate 3 kinase C; Inositol-trisphosphate 3-kinase C; InsP 3 kinase C; InsP 3-kinase C; IP3 3-kinase C; IP3 3KC; IP3K C; IP3KC; IP3KC_HUMAN; Itpkc;

Immunogens

Immunogen:

Uniprot:

Q96DU7(IP3KC_HUMAN) >>Visit HPA database.

Gene(ID):

ITPKC(80271)

Expression:

Q96DU7 IP3KC_HUMAN:

Highly expressed in pancreas, skeletal muscle, liver, placenta and weakly in kidney and brain.

Sequence:

Q96DU7 IP3KC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSEPERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTETGTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEGACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQPSTDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRVEGGSGGFSSASSFDESEDDVVAGGGGASDPEDRSGSKPWKKLKTVLKYSPFVVSFRKHYPWVQLSGHAGNFQAGEDGRILKRFCQCEQRSLEQLMKDPLRPFVPAYYGMVLQDGQTFNQMEDLLADFEGPSIMDCKMGSRTYLEEELVKARERPRPRKDMYEKMVAVDPGAPTPEEHAQGAVTKPRYMQWRETMSSTSTLGFRIEGIKKADGTCNTNFKKTQALEQVTKVLEDFVDGDHVILQKYVACLEELREALEISPFFKTHEVVGSSLLFVHDHTGLAKVWMIDFGKTVALPDHQTLSHRLPWAEGNREDGYLWGLDNMICLLQGLAQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Dog

Bovine

Sheep

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96DU7 As Substrate

Q96DU7

Site	PTM Type	Source
R7	Methylation	Uniprot
S9	Phosphorylation	Uniprot
R30	Methylation	Uniprot
S84	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
S190	Phosphorylation	Uniprot
K224	Ubiquitination	Uniprot
S322	Phosphorylation	Uniprot
T333	Phosphorylation	Uniprot
T336	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
S378	Phosphorylation	Uniprot
Y398	Phosphorylation	Uniprot
S404	Phosphorylation	Uniprot
Y456	Phosphorylation	Uniprot
Y457	Phosphorylation	Uniprot
K499	Ubiquitination	Uniprot
T566	Phosphorylation	Uniprot
K569	Ubiquitination	Uniprot
K579	Ubiquitination	Uniprot

Research Backgrounds

Q96DU7_IP3KC_HUMAN

Function:

Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles actively between nucleus and cytoplasm with both nuclear import and nuclear export activity.

Tissue Specificity:

Highly expressed in pancreas, skeletal muscle, liver, placenta and weakly in kidney and brain.

Family&Domains:

Belongs to the inositol phosphokinase (IPK) family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IP3KC Antibody - #DF3397