DLX4 Antibody | Affinity Biosciences

WB
Cites

1/2

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using DLX4 Antibody at 1/1000 dilution.

Product:	DLX4 Antibody
Catalog:	DF3387
Description:	Rabbit polyclonal antibody to DLX4
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Sheep, Dog
Mol.Wt.:	34 KD; 26kD(Calculated).
Uniprot:	Q92988
RRID:	AB_2835769

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(88%), Sheep(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

DLX4 Antibody detects endogenous levels of total DLX4.

RRID:

AB_2835769
Cite Format: Affinity Biosciences Cat# DF3387, RRID:AB_2835769.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta protein 1; BP1; Distal less homeo box 7; Distal less homeo box 9; distal-less homeobox 4; DLX4; DLX4_HUMAN; DLX7; DLX8; DLX9; Homeobox protein DLX-4; Homeobox protein DLX-7; Homeobox protein DLX-8; Homeobox protein DLX4;

Immunogens

Immunogen:

Uniprot:

Q92988(DLX4_HUMAN) >>Visit HPA database.

Gene(ID):

DLX4(1748)

Expression:

Q92988 DLX4_HUMAN:

Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver.

Sequence:

Q92988 DLX4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTSLPCPLPGRDASKAVFPDLAPVPSVAAAYPLGLSPTTAASPNLSYSRPYGHLLSYPYTEPANPGDSYLSCQQPAALSQPLCGPAEHPQELEADSEKPRLSPEPSERRPQAPAKKLRKPRTIYSSLQLQHLNQRFQHTQYLALPERAQLAAQLGLTQTQVKIWFQNKRSKYKKLLKQNSGGQEGDFPGRTFSVSPCSPPLPSLWDLPKAGTLPTSGYGNSFGAWYQHHSSDVLASPQMM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92988 As Substrate

Q92988

Site	PTM Type	Source
T2	Phosphorylation	Uniprot
S3	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S198	Phosphorylation	Uniprot

Research Backgrounds

Q92988_DLX4_HUMAN

Function:

May play a role in determining the production of hemoglobin S. May act as a repressor. During embryonic development, plays a role in palatogenesis.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver.

Family&Domains:

Belongs to the distal-less homeobox family.

References

1). Identification and validation of DLX4 as a prognostic and diagnostic biomarker for clear cell renal cell carcinoma. Oncology Letters, 2023 (PubMed: 36936018) [IF=2.9]

Application: IHC Species: Human Sample: ccRCC tissues

Figure 5. High expression levels of DLX4 in ccRCC tissues. (A and B) Reverse transcription-quantitative PCR revealed high expression levels of DLX4 mRNA in ccRCC tissues compared with normal adjacent tissue. (C and D) Immunohistochemistry showed high expression levels of DLX4 protein in ccRCC tissues compared with normal adjacent tissue. Scale bar for entire images and local magnification images were 100 and 50 µm, respectively. ccRCC, clear cell renal cell carcinoma; DLX, distal-less homeobox. **P

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DLX4 Antibody - #DF3387