BRI3B Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	BRI3B Antibody
Catalog:	DF3376
Description:	Rabbit polyclonal antibody to BRI3B
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine
Mol.Wt.:	27 KD; 28kD(Calculated).
Uniprot:	Q8WY22
RRID:	AB_2835758

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(88%)

Clonality:

Polyclonal

Specificity:

BRI3B Antibody detects endogenous levels of total BRI3B.

RRID:

AB_2835758
Cite Format: Affinity Biosciences Cat# DF3376, RRID:AB_2835758.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BNAS1; BRI3 binding protein; BRI3-binding protein; BRI3B_HUMAN; Bri3bp; Cervical cancer 1 proto oncogene binding protein KG19; Cervical cancer 1 proto-oncogene-binding protein KG19; cervical cancer oncogene binding protein; HCCR-2; HCCR1; HCCRBP-1; I3 binding protein; I3-binding protein; KG19;

Immunogens

Immunogen:

Uniprot:

Q8WY22(BRI3B_HUMAN) >>Visit HPA database.

Gene(ID):

BRI3BP(140707)

Expression:

Q8WY22 BRI3B_HUMAN:

Most abundantly expressed in brain, liver and kidney (PubMed:11860200). Overexpressed in leukemia and lymphoma cell lines, as well as in various carcinomas (PubMed:17943721).

Sequence:

Q8WY22 BRI3B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGARASGGPLARAGLLLLLLLLLLLGLLAPGAQGARGRGGAEKNSYRRTVNTFSQSVSSLFGEDNVRAAQKFLARLTERFVLGVDMFVETLWKVWTELLDVLGLDVSNLSQYFSPASVSSSPARALLLVGVVLLAYWFLSLTLGFTFSVLHVVFGRFFWIVRVVLFSMSCVYILHKYEGEPENAVLPLCFVVAVYFMTGPMGFYWRSSPSGPSNPSNPSVEEKLEHLEKQVRLLNIRLNRVLESLDRSKDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WY22 As Substrate

Q8WY22

Site	PTM Type	Source
T49	Phosphorylation	Uniprot
K71	Ubiquitination	Uniprot
K229	Acetylation	Uniprot
K229	Ubiquitination	Uniprot
S248	Phosphorylation	Uniprot

Research Backgrounds

Q8WY22_BRI3B_HUMAN

Function:

Involved in tumorigenesis and may function by stabilizing p53/TP53.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein.

Tissue Specificity:

Most abundantly expressed in brain, liver and kidney. Overexpressed in leukemia and lymphoma cell lines, as well as in various carcinomas.

Subunit Structure:

Interacts with LETMD1. Interacts with BRI3.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

BRI3B Antibody - #DF3376