CNKR2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from colo236(heat-shock treatment), using CNKR2 Antibody at 1/1000 dilution.

Product:	CNKR2 Antibody
Catalog:	DF3375
Description:	Rabbit polyclonal antibody to CNKR2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	117 KD; 118kD(Calculated).
Uniprot:	Q8WXI2
RRID:	AB_2835757

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

CNKR2 Antibody detects endogenous levels of total CNKR2.

RRID:

AB_2835757
Cite Format: Affinity Biosciences Cat# DF3375, RRID:AB_2835757.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CNK2; CNKSR2; Connector enhancer of kinase suppressor of Ras 2; Connector enhancer of KSR2; KIAA0902; KSR2; RP11 450P7.2;

Immunogens

Immunogen:

Uniprot:

Q8WXI2(CNKR2_HUMAN) >>Visit HPA database.

Gene(ID):

CNKSR2(22866)

Sequence:

Q8WXI2 CNKR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQLLRITHQELEDLGVSRIGHQELILEAVDLLCALNYGLETENLKTLSHKLNASAKNLQNFITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLAWLDRSPFAAVTDYSVTRNNVIQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQSAHLEVIQLANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVVGWQLKNLVNALREDPSGVILTLKKRPQSMLTSAPALLKNMRWKPLALQPLIPRSPTSSVATPSSTISTPTKRDSSALQDLYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKPVHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGKSKRRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFKIDRASECRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYWSESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPLEDSVFSDSAAISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGGKPRSFTLPRDSGFNHCCLNAPVSACDPQDDVQPPEVEEEEEEEEEEGEAAGENIGEKSESREEKLGDSLQDLYRALEQASLSPLGEHRISTKMEYKLSFIKRCNDPVMNEKLHRLRILKSTLKAREGEVAIIDKVLDNPDLTSKEFQQWKQMYLDLFLDICQNTTSNDPLSISSEVDVITSSLAHTHSYIETHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXI2 As Substrate

Q8WXI2

Site	PTM Type	Source
T292	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot
S325	Phosphorylation	Uniprot
S388	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
T433	Phosphorylation	Uniprot
Y434	Phosphorylation	Uniprot
Y452	Phosphorylation	Uniprot
S467	Phosphorylation	Uniprot
S473	Phosphorylation	Uniprot
S564	Phosphorylation	Uniprot
T694	Phosphorylation	Uniprot
S756	Phosphorylation	Uniprot
S808	Phosphorylation	Uniprot
Y821	Phosphorylation	Uniprot
S908	Phosphorylation	Uniprot

Research Backgrounds

Q8WXI2_CNKR2_HUMAN

Function:

May function as an adapter protein or regulator of Ras signaling pathways.

PTMs:

Phosphorylated on tyrosine.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Subunit Structure:

Interacts with RAF1, RAB2L and RAL GTPase proteins.

Family&Domains:

Belongs to the CNKSR family.

Restrictive clause

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For Research Use Only.
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CNKR2 Antibody - #DF3375