GRK7 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HeLa cells using GRK7 antibody.

Product:	GRK7 Antibody
Catalog:	DF3370
Description:	Rabbit polyclonal antibody to GRK7
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	62 KD; 62kD(Calculated).
Uniprot:	Q8WTQ7
RRID:	AB_2835752

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GRK7 Antibody detects endogenous levels of total GRK7.

RRID:

AB_2835752
Cite Format: Affinity Biosciences Cat# DF3370, RRID:AB_2835752.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein coupled receptor kinase 7; G protein coupled receptor kinase GRK7; G protein-coupled receptor kinase 7; G protein-coupled receptor kinase GRK7; GPRK 7; GPRK7; GRK 7; GRK7; GRK7_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q8WTQ7(GRK7_HUMAN) >>Visit HPA database.

Gene(ID):

GRK7(131890)

Expression:

Q8WTQ7 GRK7_HUMAN:

Retinal cones, outer and inner segments.

Sequence:

Q8WTQ7 GRK7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVDMGALDNLIANTAYLQARKPSDCDSKELQRRRRSLALPGLQGCAELRQKLSLNFHSLCEQQPIGRRLFRDFLATVPTFRKAATFLEDVQNWELAEEGPTKDSALQGLVATCASAPAPGNPQPFLSQAVATKCQAATTEEERVAAVTLAKAEAMAFLQEQPFKDFVTSAFYDKFLQWKLFEMQPVSDKYFTEFRVLGKGGFGEVCAVQVKNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVSSPFIVSLAYAFESKTHLCLVMSLMNGGDLKFHIYNVGTRGLDMSRVIFYSAQIACGMLHLHELGIVYRDMKPENVLLDDLGNCRLSDLGLAVEMKGGKPITQRAGTNGYMAPEILMEKVSYSYPVDWFAMGCSIYEMVAGRTPFKDYKEKVSKEDLKQRTLQDEVKFQHDNFTEEAKDICRLFLAKKPEQRLGSREKSDDPRKHHFFKTINFPRLEAGLIEPPFVPDPSVVYAKDIAEIDDFSEVRGVEFDDKDKQFFKNFATGAVPIAWQEEIIETGLFEELNDPNRPTGCEEGNSSKSGVCLLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WTQ7 As Substrate

Q8WTQ7

Site	PTM Type	Enzyme	Source
S23	Phosphorylation	P17612 (PRKACA)	Uniprot
S36	Phosphorylation	P17612 (PRKACA) , P17252 (PRKCA)	Uniprot
T85	Phosphorylation		Uniprot
K229	Acetylation		Uniprot
K230	Acetylation		Uniprot
K234	Acetylation		Uniprot
K433	Acetylation		Uniprot
S490	Phosphorylation	Q8WTQ7 (GRK7)	Uniprot

PTMs - Q8WTQ7 As Enzyme

Q8WTQ7

Substrate	Site	Source
Q8WTQ7 (GRK7)	S490	Uniprot

Research Backgrounds

Q8WTQ7_GRK7_HUMAN

Function:

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).

PTMs:

Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.

Subcellular Location:

Membrane>Lipid-anchor.

Tissue Specificity:

Retinal cones, outer and inner segments.

Subunit Structure:

Interacts (when prenylated) with PDE6D; this promotes release from membranes.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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GRK7 Antibody - #DF3370