BCOR Antibody - #DF3520

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Product: BCOR Antibody
Catalog: DF3520
Description: Rabbit polyclonal antibody to BCOR
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 192 KD; 192kD(Calculated).
Uniprot: Q6W2J9
RRID: AB_2835740

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BCOR Antibody detects endogenous levels of total BCOR.
RRID:
AB_2835740
Cite Format: Affinity Biosciences Cat# DF3520, RRID:AB_2835740.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5830466J11Rik; 8430401K06Rik; ANOP 2; ANOP2; BCL 6 co repressor; BCL 6 corepressor; BCL 6 interacting corepressor; BCL-6 corepressor; BCL6 co repressor; BCL6 corepressor; BCL6 interacting corepressor; BCoR; BCOR_HUMAN; FLJ20285; FLJ38041; KIAA1575; MAA 2; MAA2; MCOPS 2; MCOPS2; MGC131961; MGC71031;

Immunogens

Immunogen:
Uniprot:

Q6W2J9(BCOR_HUMAN) >>Visit HPA database.

Gene(ID):
BCOR(54880)
Expression:
Q6W2J9 BCOR_HUMAN:

Ubiquitously expressed.

Sequence:
MLSATPLYGNVHSWMNSERVRMCGASEDRKILVNDGDASKARLELREENPLNHNVVDASTAHRIDGLAALSMDRTGLIREGLRVPGNIVYSSLCGLGSEKGREAATSTLGGLGFSSERNPEMQFKPNTPETVEASAVSGKPPNGFSAIYKTPPGIQKSAVATAEALGLDRPASDKQSPLNINGASYLRLPWVNPYMEGATPAIYPFLDSPNKYSLNMYKALLPQQSYSLAQPLYSPVCTNGERFLYLPPPHYVGPHIPSSLASPMRLSTPSASPAIPPLVHCADKSLPWKMGVSPGNPVDSHAYPHIQNSKQPRVPSAKAVTSGLPGDTALLLPPSPRPSPRVHLPTQPAADTYSEFHKHYARISTSPSVALSKPYMTVSSEFPAARLSNGKYPKAPEGGEGAQPVPGHARKTAVQDRKDGSSPPLLEKQTVTKDVTDKPLDLSSKVVDVDASKADHMKKMAPTVLVHSRAGSGLVLSGSEIPKETLSPPGNGCAIYRSEIISTAPSSWVVPGPSPNEENNGKSMSLKNKALDWAIPQQRSSSCPRMGGTDAVITNVSGSVSSAGRPASASPAPNANADGTKTSRSSVETTPSVIQHVGQPPATPAKHSSSTSSKGAKASNPEPSFKANENGLPPSSIFLSPNEAFRSPPIPYPRSYLPYPAPEGIAVSPLSLHGKGPVYPHPVLLPNGSLFPGHLAPKPGLPYGLPTGRPEFVTYQDALGLGMVHPMLIPHTPIEITKEEKPERRSRSHERARYEDPTLRNRFSEILETSSTKLHPDVPTDKNLKPNPNWNQGKTVVKSDKLVYVDLLREEPDAKTDTNVSKPSFAAESVGQSAEPPKPSVEPALQQHRDFIALREELGRISDFHETYTFKQPVFTVSKDSVLAGTNKENLGLPVSTPFLEPPLGSDGPAVTFGKTQEDPKPFCVGSAPPSVDVTPTYTKDGADEAESNDGKVLKPKPSKLAKRIANSAGYVGDRFKCVTTELYADSSQLSREQRALQMEGLQEDSILCLPAAYCERAMMRFSELEMKEREGGHPATKDSEMCKFSPADWERLKGNQDKKPKSVTLEEAIAEQNESERCEYSVGNKHRDPFEAPEDKDLPVEKYFVERQPVSEPPADQVASDMPHSPTLRVDRKRKVSGDSSHTETTAEEVPEDPLLKAKRRRVSKDDWPEREMTNSSSNHLEDPHYSELTNLKVCIELTGLHPKKQRHLLHLRERWEQQVSAADGKPGRQSRKEVTQATQPEAIPQGTNITEEKPGRKRAEAKGNRSWSEESLKPSDNEQGLPVFSGSPPMKSLSSTSAGGKKQAQPSCAPASRPPAKQQKIKENQKTDVLCADEEEDCQAASLLQKYTDNSEKPSGKRLCKTKHLIPQESRRGLPLTGEYYVENADGKVTVRRFRKRPEPSSDYDLSPAKQEPKPFDRLQQLLPASQSTQLPCSSSPQETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKICDVNHRDNAGYCALHEACARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLATYSGRTIMKMTHSELMEKFLTDYLNDLQGRNDDDASGTWDFYGSSVCEPDDESGYDVLANPPGPEDQDDDDDAYSDVFEFEFSETPLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIFRCNFPNVEIVTIAEAEFYRQVSASLLFSCSKDLEAFNPESKELLDLVEFTNEIQTLLGSSVEWLHPSDLASDNYW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6W2J9 As Substrate

Site PTM Type Enzyme
K30 Ubiquitination
K40 Ubiquitination
Y90 Phosphorylation
S98 Phosphorylation
K100 Ubiquitination
T106 Phosphorylation
S116 Phosphorylation
T128 Phosphorylation
Y149 Phosphorylation
K150 Ubiquitination
T151 Phosphorylation
K157 Ubiquitination
K175 Ubiquitination
S177 Phosphorylation
R188 Methylation
T200 Phosphorylation
Y227 Phosphorylation
S228 Phosphorylation
S235 Phosphorylation
R243 Methylation
T269 Phosphorylation
S273 Phosphorylation
K290 Ubiquitination
S294 Phosphorylation
K311 Ubiquitination
S336 Phosphorylation
R338 Methylation
S340 Phosphorylation
R342 Methylation
Y361 Phosphorylation
S365 O-Glycosylation
S365 Phosphorylation
S367 Phosphorylation
S369 Phosphorylation
K374 Acetylation
T378 Phosphorylation
S389 Phosphorylation
K392 Acetylation
K412 Ubiquitination
T413 Phosphorylation
S422 Phosphorylation
S423 Phosphorylation
K434 Ubiquitination
K439 Ubiquitination
K446 Ubiquitination
K454 Ubiquitination
K460 Ubiquitination
T464 Phosphorylation
S469 Phosphorylation
S473 Phosphorylation
S478 Phosphorylation
S480 Phosphorylation
S488 Phosphorylation
S503 Phosphorylation
K530 Sumoylation
K530 Ubiquitination
T555 Phosphorylation
S560 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
T604 Phosphorylation
S641 Phosphorylation
R647 Methylation
R655 Methylation
S669 Phosphorylation
S765 Phosphorylation
K774 Ubiquitination
K786 Sumoylation
K786 Ubiquitination
K795 Acetylation
K795 Ubiquitination
K802 Acetylation
K816 Acetylation
K823 Acetylation
K823 Ubiquitination
K839 Ubiquitination
K872 Ubiquitination
K880 Ubiquitination
K889 Ubiquitination
T917 Phosphorylation
S928 Phosphorylation
S932 Phosphorylation
T936 Phosphorylation
Y972 Phosphorylation
Y985 Phosphorylation
K1029 Ubiquitination
K1045 Ubiquitination
S1047 Phosphorylation
S1064 Phosphorylation
K1104 Ubiquitination
S1122 Phosphorylation
S1127 Phosphorylation
T1129 Phosphorylation
S1139 Phosphorylation
S1142 Phosphorylation
S1143 Phosphorylation
T1145 Phosphorylation
T1147 Phosphorylation
T1148 Phosphorylation
S1166 Phosphorylation
S1223 Phosphorylation
S1269 Phosphorylation
S1271 Phosphorylation
S1278 Phosphorylation
S1288 Phosphorylation
S1290 Phosphorylation
K1304 Acetylation
K1323 Methylation
K1325 Methylation
K1329 Ubiquitination
K1349 Ubiquitination
K1366 Ubiquitination
S1404 Phosphorylation
S1405 Phosphorylation
S1410 Phosphorylation
K1460 Sumoylation
K1460 Ubiquitination
Y1519 Phosphorylation
S1526 Phosphorylation
T1531 Phosphorylation

Research Backgrounds

Function:

Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with BCL6; the interaction is direct. Forms ternary complexes with BCL6 and SMRT/NCOR2 on selected target genes promoters; potently repress expression. Can interact with HDAC1, HDAC3 and HDAC5. Interacts with PCGF1; the interaction is direct. Interacts with KDM2B. Component of an approximately 800 kDa repressive BCOR complex at least composed of BCOR, RYBP, PCGF1, RING1, RNF2/RING2, KDM2B and SKP1. Interacts with CPNE4 (via VWFA domain) (By similarity). Isoform 1 may interact with MLLT3/AF9 (By similarity).

Family&Domains:

Belongs to the BCOR family.

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