Product: PRDM12 Antibody
Catalog: DF3435
Description: Rabbit polyclonal antibody to PRDM12
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Rabbit, Dog, Chicken
Mol.Wt.: 40 KD; 40kD(Calculated).
Uniprot: Q9H4Q4
RRID: AB_2835735

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PRDM12 Antibody detects endogenous levels of total PRDM12.
RRID:
AB_2835735
Cite Format: Affinity Biosciences Cat# DF3435, RRID:AB_2835735.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gm998; PFM 9; PFM9; PR domain containing 12; PR domain containing protein 12; PR domain zinc finger protein 12; PR domain-containing protein 12; PRD12_HUMAN; PRDM 12; PRDM12;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9H4Q4 PRD12_HUMAN:

Not found in adult tissues except in dorsal root ganglia.

Sequence:
MMGSVLPAEALVLKTGLKAPGLALAEVITSDILHSFLYGRWRNVLGEQLFEDKSHHASPKTAFTAEVLAQSFSGEVQKLSSLVLPAEVIIAQSSIPGEGLGIFSKTWIKAGTEMGPFTGRVIAPEHVDICKNNNLMWEVFNEDGTVRYFIDASQEDHRSWMTYIKCARNEQEQNLEVVQIGTSIFYKAIEMIPPDQELLVWYGNSHNTFLGIPGVPGLEEDQKKNKHEDFHPADSAAGPAGRMRCVICHRGFNSRSNLRSHMRIHTLDKPFVCRFCNRRFSQSSTLRNHVRLHTGERPYKCQVCQSAYSQLAGLRAHQKSARHRPPSTALQAHSPALPAPHAHAPALAAAAAAAAAAAAHHLPAMVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
75
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4Q4 As Substrate

Site PTM Type Enzyme
S159 Phosphorylation
R259 Methylation
S260 Phosphorylation
R263 Methylation
K269 Acetylation
K269 Methylation
T294 Phosphorylation

Research Backgrounds

Function:

Involved in the positive regulation of histone H3-K9 dimethylation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Not found in adult tissues except in dorsal root ganglia.

Family&Domains:

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.