NT5C3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells using NT5C3 antibody.

Product:	NT5C3 Antibody
Catalog:	DF3430
Description:	Rabbit polyclonal antibody to NT5C3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Sheep
Mol.Wt.:	38 KD; 38kD(Calculated).
Uniprot:	Q9H0P0
RRID:	AB_2835730

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Horse(100%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

NT5C3 Antibody detects endogenous levels of total NT5C3.

RRID:

AB_2835730
Cite Format: Affinity Biosciences Cat# DF3430, RRID:AB_2835730.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5NT3A_HUMAN; cN III; cN-III; cNIII; Cytosolic 5' nucleotidase 3; Cytosolic 5' nucleotidase III; Cytosolic 5''-nucleotidase 3; Cytosolic 5''-nucleotidase 3A; Cytosolic 5''-nucleotidase III; Nt5c3a; p36; P5''N-1; P5'N 1; P5'N1; P5N 1; P5N-1; P5N1; PN I; PN-I; PNI; PSN1; Pyrimidine 5' nucleotidase 1; Pyrimidine 5''-nucleotidase 1; UMPH; UMPH1; Uridine 5' monophosphate hydrolase 1; Uridine 5''-monophosphate hydrolase 1;

Immunogens

Immunogen:

Uniprot:

Q9H0P0(5NT3A_HUMAN) >>Visit HPA database.

Gene(ID):

NT5C3A(51251)

Expression:

Q9H0P0 5NT3A_HUMAN:

Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.

Sequence:

Q9H0P0 5NT3A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRAPSMDRAAVARVGAVASASVCALVAGVVLAQYIFTLKRKTGRKTKIIEMMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGALRNTEYFNQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Pig

Chicken

Bovine

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H0P0 As Substrate

Q9H0P0

Site	PTM Type	Source
K47	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
K77	Ubiquitination	Uniprot
K128	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K225	Ubiquitination	Uniprot
S228	Phosphorylation	Uniprot
T236	Phosphorylation	Uniprot
K243	Ubiquitination	Uniprot
K252	Ubiquitination	Uniprot
T260	Phosphorylation	Uniprot
K267	Ubiquitination	Uniprot
S278	Phosphorylation	Uniprot
K296	Ubiquitination	Uniprot
R303	Methylation	Uniprot
K334	Ubiquitination	Uniprot

Research Backgrounds

Q9H0P0_5NT3A_HUMAN

Function:

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.

Subcellular Location:

Cytoplasm.

Endoplasmic reticulum.

Tissue Specificity:

Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the pyrimidine 5'-nucleotidase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NT5C3 Antibody - #DF3430