SMRC2 Antibody - #DF3363
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3363, RRID:AB_2835721.
Fold/Unfold
BAF 170; BAF170; BRG1 associated factor 170; BRG1-associated factor 170; Chromatin remodeling complex BAF170 subunit; CRACC 2; CRACC2; Mammalian chromatin remodeling complex BRG1 associated factor 170; Rsc 8; Rsc8; SMARCC 2; Smarcc2; SMRC2_HUMAN; SWI/SNF complex 170 kDa subunit; SWI/SNF complex subunit SMARCC2; SWI/SNF related matrix associated actin dependent regulator of chromatin c2; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2; SWI3 like protein;
Immunogens
- Q8TAQ2 SMRC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVRKKDGGPNVKYYEAADTVTQFDNVRLWLGKNYKKYIQAEPPTNKSLSSLVVQLLQFQEEVFGKHVSNAPLTKLPIKCFLDFKAGGSLCHILAAAYKFKSDQGWRRYDFQNPSRMDRNVEMFMTIEKSLVQNNCLSRPNIFLCPEIEPKLLGKLKDIIKRHQGTVTEDKNNASHVVYPVPGNLEEEEWVRPVMKRDKQVLLHWGYYPDSYDTWIPASEIEASVEDAPTPEKPRKVHAKWILDTDTFNEWMNEEDYEVNDDKNPVSRRKKISAKTLTDEVNSPDSDRRDKKGGNYKKRKRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVPNVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQTHHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATREWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLEDSEASLGPLAYQPIPFSQSGNPVMSTVAFLASVVDPRVASAAAKSALEEFSKMKEEVPTALVEAHVRKVEEAAKVTGKADPAFGLESSGIAGTTSDEPERIEESGNDEARVEGQATDEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKGKEGDSEKESEKSDGDPIVDPEKEKEPKEGQEEVLKEVVESEGERKTKVERDIGEGNLSTAAAAALAAAAVKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREREALEYQRQQLLADRQAFHMEQLKYAEMRARQQHFQQMHQQQQQPPPALPPGSQPIPPTGAAGPPAVHGLAVAPASVVPAPAGSGAPPGSLGPSEQIGQAGSTAGPQQQQPAGAPQPGAVPPGVPPPGPHGPSPFPNQQTPPSMMPGAVPGSGHPGVAGNAPLGLPFGMPPPPPPPAPSIIPFGSLADSISINLPAPPNLHGHHHHLPFAPGTLPPPNLPVSMANPLHPNLPATTTMPSSLPLGPGLGSAAAQSPAIVAAVQGNLLPSASPLPDPGTPLPPDPTAPSPGTVTPVPPPQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TAQ2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K37 | Ubiquitination | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| C145 | S-Nitrosylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K326 | Acetylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| T400 | Phosphorylation | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| T579 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| T583 | Phosphorylation | Uniprot | |
| K622 | Ubiquitination | Uniprot | |
| C635 | S-Nitrosylation | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| K704 | Sumoylation | Uniprot | |
| K704 | Ubiquitination | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| T726 | Phosphorylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| K769 | Acetylation | Uniprot | |
| K795 | Acetylation | Uniprot | |
| S806 | Phosphorylation | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| K836 | Ubiquitination | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| K872 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| K885 | Ubiquitination | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| R931 | Methylation | Uniprot | |
| K940 | Ubiquitination | Uniprot | |
| S969 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
Research Backgrounds
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (By similarity).
Nucleus.
Ubiquitously expressed.
Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B) and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific (Probable). Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin. May also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. Interacts with SMARD1. Interacts with KDM6B (By similarity). Interaction with RCOR1. Interacts with DPF2. Interacts with ERCC6.
Belongs to the SMARCC family.
Research Fields
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
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