DCLK2 Antibody - #DF3349

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Product: DCLK2 Antibody
Catalog: DF3349
Description: Rabbit polyclonal antibody to DCLK2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 83 KD; 84kD(Calculated).
Uniprot: Q8N568
RRID: AB_2835716

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DCLK2 Antibody detects endogenous levels of total DCLK2.
RRID:
AB_2835716
Cite Format: Affinity Biosciences Cat# DF3349, RRID:AB_2835716.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; DCAMK L2; DCAMKL 2; DCDC 3; DCDC 3B; DCDC3; DCDC3B; DCK 2; DCK2; DCLK 2; Dclk2; DCLK2_HUMAN; DKFZp761I032; Doublecortin and CaM kinase like 2; Doublecortin domain-containing protein 3B; Doublecortin like and CAM kinase like 2; Doublecortin like kinase 2; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2; MGC45428; Serine/threonine protein kinase DCLK2; Serine/threonine-protein kinase DCLK2;

Immunogens

Immunogen:
Uniprot:

Q8N568(DCLK2_HUMAN) >>Visit HPA database.

Gene(ID):
DCLK2(166614)
Expression:
Q8N568 DCLK2_HUMAN:

Expressed in the brain, heart and eyes.

Sequence:
MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKNINPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDDFVLDHSECRVLKSSYSRSSAVKYSGSKSPGPSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAHGRSSSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISPVPPSVEEIPVPGEAVPAPTPPESPTPHPPPAAPGGERAGTWRRHRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N568 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
S47 Phosphorylation
S51 Phosphorylation
T61 Phosphorylation
Y83 Phosphorylation
S93 Phosphorylation
T173 Phosphorylation
S182 Phosphorylation
S185 Phosphorylation
S308 Phosphorylation
S312 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
S327 Phosphorylation
T331 Phosphorylation
S337 Phosphorylation
S338 Phosphorylation
S340 Phosphorylation
S344 Phosphorylation
S347 Phosphorylation
S355 Phosphorylation
S360 Phosphorylation
S362 Phosphorylation
S375 Phosphorylation
K409 Ubiquitination
Y505 Phosphorylation
S682 Phosphorylation
T685 Phosphorylation

Research Backgrounds

Function:

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Colocalizes with microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, heart and eyes.

Subunit Structure:

Binds to and stabilizes microtubules. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin.

Family&Domains:

The doublecortin domains are involved in the colocalization with microtubules.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Restrictive clause

 

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