FUK Antibody | Affinity Biosciences

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Western blot analysis of extracts from COS-7 cells, treated with insulin (0.

Product:	FUK Antibody
Catalog:	DF3347
Description:	Rabbit polyclonal antibody to FUK
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	110 KD; 118kD(Calculated).
Uniprot:	Q8N0W3
RRID:	AB_2835714

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

FUK Antibody detects endogenous levels of total FUK.

RRID:

AB_2835714
Cite Format: Affinity Biosciences Cat# DF3347, RRID:AB_2835714.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1110046B12Rik; FLJ39408; Fucokinase; FUK; FUK_HUMAN; L-fucose kinase;

Immunogens

Immunogen:

Uniprot:

Q8N0W3(FCSK_HUMAN) >>Visit HPA database.

Gene(ID):

FUK(197258)

Expression:

Q8N0W3 FCSK_HUMAN:

Expressed in fibroblasts.

Sequence:

Q8N0W3 FCSK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQPKGVDWTVIILTCQYKDSVQVFQRELEVRQKREQIPAGTLLLAVEDPEKRVGSGGATLNALLVAAEHLSARAGFTVVTSDVLHSAWILILHMGRDFPFDDCGRAFTCLPVENPEAPVEALVCNLDCLLDIMTYRLGPGSPPGVWVCSTDMLLSVPANPGISWDSFRGARVIALPGSPAYAQNHGVYLTDPQGLVLDIYYQGTEAEIQRCVRPDGRVPLVSGVVFFSVETAERLLATHVSPPLDACTYLGLDSGARPVQLSLFFDILHCMAENVTREDFLVGRPPELGQGDADVAGYLQSARAQLWRELRDQPLTMAYVSSGSYSYMTSSASEFLLSLTLPGAPGAQIVHSQVEEQQLLAAGSSVVSCLLEGPVQLGPGSVLQHCHLQGPIHIGAGCLVTGLDTAHSKALHGRELRDLVLQGHHTRLHGSPGHAFTLVGRLDSWERQGAGTYLNVPWSEFFKRTGVRAWDLWDPETLPAEYCLPSARLFPVLHPSRELGPQDLLWMLDHQEDGGEALRAWRASWRLSWEQLQPCLDRAATLASRRDLFFRQALHKARHVLEARQDLSLRPLIWAAVREGCPGPLLATLDQVAAGAGDPGVAARALACVADVLGCMAEGRGGLRSGPAANPEWMRPFSYLECGDLAAGVEALAQERDKWLSRPALLVRAARHYEGAGQILIRQAVMSAQHFVSTEQVELPGPGQWVVAECPARVDFSGGWSDTPPLAYELGGAVLGLAVRVDGRRPIGARARRIPEPELWLAVGPRQDEMTVKIVCRCLADLRDYCQPHAPGALLKAAFICAGIVHVHSELQLSEQLLRTFGGGFELHTWSELPHGSGLGTSSILAGTALAALQRAAGRVVGTEALIHAVLHLEQVLTTGGGWQDQVGGLMPGIKVGRSRAQLPLKVEVEEVTVPEGFVQKLNDHLLLVYTGKTRLARNLLQDVLRSWYARLPAVVQNAHSLVRQTEECAEGFRQGSLPLLGQCLTSYWEQKKLMAPGCEPLTVRRMMDVLAPHVHGQSLAGAGGGGFLYLLTKEPQQKEALEAVLAKTEGLGNYSIHLVEVDTQGLSLKLLGTEASTCCPFP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N0W3 As Substrate

Q8N0W3

Site	PTM Type	Source
Y18	Phosphorylation	Uniprot
K52	Ubiquitination	Uniprot
S223	Phosphorylation	Uniprot
S229	Phosphorylation	Uniprot
T232	Phosphorylation	Uniprot
K464	Ubiquitination	Uniprot
K659	Ubiquitination	Uniprot
Y729	Phosphorylation	Uniprot
K774	Ubiquitination	Uniprot
K907	Ubiquitination	Uniprot
K934	Ubiquitination	Uniprot
K994	Ubiquitination	Uniprot
T1004	Phosphorylation	Uniprot
K1040	Ubiquitination	Uniprot

Research Backgrounds

Q8N0W3_FCSK_HUMAN

Function:

Takes part in the salvage pathway for reutilization of fucose from the degradation of oligosaccharides.

Tissue Specificity:

Expressed in fibroblasts.

Family&Domains:

Belongs to the GHMP kinase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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For Research Use Only.
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FUK Antibody - #DF3347