TRIM59 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using TRIM59 Antibody.

Product:	TRIM59 Antibody
Catalog:	DF3343
Description:	Rabbit polyclonal antibody to TRIM59
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	47 KD; 47kD(Calculated).
Uniprot:	Q8IWR1
RRID:	AB_2835710

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(100%), Horse(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

TRIM59 Antibody detects endogenous levels of total TRIM59.

RRID:

AB_2835710
Cite Format: Affinity Biosciences Cat# DF3343, RRID:AB_2835710.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310035M22Rik; 2700022F13Rik; MGC129860; MGC129861; MGC26631; MRF1; RING finger protein 104; RNF104; TRI59_HUMAN; TRIM57; TRIM59; Tripartite motif containing 57; tripartite motif containing 59; Tripartite motif-containing protein 59; TSBF1; Tumor suppressor TSBF 1; Tumor suppressor TSBF-1;

Immunogens

Immunogen:

Uniprot:

Q8IWR1(TRI59_HUMAN) >>Visit HPA database.

Gene(ID):

TRIM59(286827)

Sequence:

Q8IWR1 TRI59_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHNFEEELTCPICYSIFEDPRVLPCSHTFCRNCLENILQASGNFYIWRPLRIPLKCPNCRSITEIAPTGIESLPVNFALRAIIEKYQQEDHPDIVTCPEHYRQPLNVYCLLDKKLVCGHCLTIGQHHGHPIDDLQSAYLKEKDTPQKLLEQLTDTHWTDLTHLIEKLKEQKSHSEKMIQGDKEAVLQYFKELNDTLEQKKKSFLTALCDVGNLINQEYTPQIERMKEIREQQLELMALTISLQEESPLKFLEKVDDVRQHVQILKQRPLPEVQPVEIYPRVSKILKEEWSRTEIGQIKNVLIPKMKISPKRMSCSWPGKDEKEVEFLKILNIVVVTLISVILMSILFFNQHIITFLSEITLIWFSEASLSVYQSLSNSLHKVKNILCHIFYLLKEFVWKIVSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Pig

Horse

Dog

Chicken

Sheep

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IWR1 As Substrate

Q8IWR1

Site	PTM Type	Source
K176	Ubiquitination	Uniprot
K253	Ubiquitination	Uniprot
Y278	Phosphorylation	Uniprot
K298	Ubiquitination	Uniprot
S308	Phosphorylation	Uniprot
K310	Acetylation	Uniprot

Research Backgrounds

Q8IWR1_TRI59_HUMAN

Function:

May serve as a multifunctional regulator for innate immune signaling pathways.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Subunit Structure:

Interacts with ECSIT.

Family&Domains:

Belongs to the TRIM/RBCC family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TRIM59 Antibody - #DF3343