Product: FAKD1 Antibody
Catalog: DF3315
Description: Rabbit polyclonal antibody to FAKD1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 97 KD; 97kD(Calculated).
Uniprot: Q53R41
RRID: AB_2835683

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
FAKD1 Antibody detects endogenous levels of total FAKD1.
RRID:
AB_2835683
Cite Format: Affinity Biosciences Cat# DF3315, RRID:AB_2835683.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FAKD1_HUMAN; FAST kinase domain containing protein 1; FAST kinase domain-containing protein 1; FAST kinase domains 1; FASTKD1; FLJ21901; KIAA1800; OTTHUMP00000207008; OTTHUMP00000207010;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q53R41 FAKD1_HUMAN:

Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in heart.

Sequence:
MKKTPVFLESLVTNMLRLRAICPFSWRVFQFRPISCEPLIIQMNKCTDEEQMFGFIERNKAILSEKQVGCAFDMLWKLQKQKTSLLKNAEYVRDHPQFLTLHNLATNKFKLMNDDTLVNVLYVTQQFAGEAHDPLVEALVTEAWRRLERFDIKLLSEFSSCLADQHLYFSPLMGKIADIVHRNLETTQDLSSLSVLMVNISSLISRHFQQQLVNKTELLFDTIDSSEVNVAKSIAKFLRNVRYRYQPLLERCNNVFLSNVDHLDLDSISKILSVYKFLQFNSFEFIIMAKKKLTEMIPLCNHPASFVKLFVALGPIAGPEEKKQLKSTMLLMSEDLTGEQALAVLGAMGDMESRNSCLIKRVTSVLHKHLDGYKPLELLKITQELTFLHFQRKEFFAKLRELLLSYLKNSFIPTEVSVLVRAISLLPSPHLDEVGISRIEAVLPQCDLNNLSSFATSVLRWIQHDHMYLDNMTAKQLKLLQKLDHYGRQRLQHSNSLDLLRKELKSLKGNTFPESLLEEMIATLQHFMDDINYINVGEIASFISSTDYLSTLLLDRIASVAVQQIEKIHPFTIPAIIRPFSVLNYDPPQRDEFLGTCVQHLNSYLGILDPFILVFLGFSLATLEYFPEDLLKAIFNIKFLARLDSQLEILSPSRSARVQFHLMELNRSVCLECPEFQIPWFHDRFCQQYNKGIGGMDGTQQQIFKMLAEVLGGINCVKASVLTPYYHKVDFECILDKRKKPLPYGSHNIALGQLPEMPWESNIEIVGSRLPPGAERIALEFLDSKALCRNIPHMKGKSAMKKRHLEILGYRVIQISQFEWNSMALSTKDARMDYLRECIFGEVKSCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
88
Rabbit
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53R41 As Substrate

Site PTM Type Enzyme
K60 Ubiquitination
T83 Phosphorylation
K87 Ubiquitination
Y122 Phosphorylation
T124 Phosphorylation
Y168 Phosphorylation
S170 Phosphorylation
S194 Phosphorylation
S202 Phosphorylation
S305 Phosphorylation
K360 Acetylation
K374 Ubiquitination
S405 Phosphorylation
Y406 Phosphorylation
K478 Ubiquitination
K482 Ubiquitination
Y486 Phosphorylation
S496 Phosphorylation
T546 Phosphorylation
T551 Phosphorylation
K691 Ubiquitination
Y726 Phosphorylation
K728 Ubiquitination
K737 Ubiquitination
K785 Ubiquitination
Y810 Phosphorylation
K844 Ubiquitination

Research Backgrounds

Function:

Involved in the down-regulation of mitochondrial MT-ND3 mRNA levels which leads to decreased respiratory complex I abundance and activity.

Subcellular Location:

Mitochondrion.
Note: Preferentially localizes to mitochondrial RNA granules, platforms for post-transcriptional RNA modification and ribosome assembly (PubMed:28335001).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expression detected in spleen, thymus, testis, ovary, colon, heart, smooth muscle, kidney, brain, lung, liver and white adipose tissue with highest expression in heart.

Family&Domains:

The RAP domain is essential to regulate MT-ND3 mRNA levels.

Belongs to the FAST kinase family.

Restrictive clause

 

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For Research Use Only.
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