DAK Antibody - #DF3310

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Product: DAK Antibody
Catalog: DF3310
Description: Rabbit polyclonal antibody to DAK
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 59 KD; 59kD(Calculated).
Uniprot: Q3LXA3
RRID: AB_2835678

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DAK Antibody detects endogenous levels of total DAK.
RRID:
AB_2835678
Cite Format: Affinity Biosciences Cat# DF3310, RRID:AB_2835678.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent dihydroxyacetone kinase; Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing); DAK; DHA kinase; Dha kinase/FMN cyclase; DHAK_HUMAN; Dihydroxyacetone kinase; Dihydroxyacetone kinase 2; Dihydroxyacetone kinase 2 homolog (S. cerevisiae); Dihydroxyacetone kinase 2 homolog (yeast); FAD AMP lyase (cyclic FMN forming); FAD AMP lyase (cyclizing); FAD-AMP lyase (cyclic FMN forming); FAD-AMP lyase (cyclizing); FMN cyclase; Glycerone kinase;

Immunogens

Immunogen:
Uniprot:

Q3LXA3(TKFC_HUMAN) >>Visit HPA database.

Gene(ID):
TKFC(26007)
Expression:
Q3LXA3 TKFC_HUMAN:

Detected in erythrocytes (at protein level).

Sequence:
MTSKKLVNSVAGCADDALAGLVACNPNLQLLQGHRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFTSPAVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAREQARAEGIPVEMVVIGDDSAFTVLKKAGRRGLCGTVLIHKVAGALAEAGVGLEEIAKQVNVVAKAMGTLGVSLSSCSVPGSKPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLMLDHMTNTTNASHVPVQPGSSVVMMVNNLGGLSFLELGIIADATVRSLEGRGVKIARALVGTFMSALEMPGISLTLLLVDEPLLKLIDAETTAAAWPNVAAVSITGRKRSRVAPAEPQEAPDSTAAGGSASKRMALVLERVCSTLLGLEEHLNALDRAAGDGDCGTTHSRAARAIQEWLKEGPPPASPAQLLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKAKTSLPAWSAAMDAGLEAMQKYGKAAPGDRTMLDSLWAAGQELQAWKSPGADLLQVLTKAVKSAEAAAEATKNMEAGAGRASYISSARLEQPDPGAVAAAAILRAILEVLQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q3LXA3 As Substrate

Site PTM Type Enzyme
K46 Ubiquitination
R115 Methylation
S343 Phosphorylation
S350 Phosphorylation
S363 Phosphorylation
S369 Phosphorylation
S371 Phosphorylation
K372 Acetylation
K372 Ubiquitination
C404 S-Nitrosylation
K420 Ubiquitination
S427 Phosphorylation
S436 Phosphorylation
K465 Ubiquitination
S511 Phosphorylation
K522 Ubiquitination
K525 Ubiquitination
S545 Phosphorylation
Y546 Phosphorylation
S575 Phosphorylation

Research Backgrounds

Function:

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.

Tissue Specificity:

Detected in erythrocytes (at protein level).

Subunit Structure:

Homodimer (By similarity). Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection.

Family&Domains:

DhaK and DhaL domains have differential roles, individually DhaK is inactive and DhaL displays cyclase but not kinase activity.

Belongs to the dihydroxyacetone kinase (DAK) family.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

Restrictive clause

 

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