PFKFB1/4 Antibody - #DF3307

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Product: PFKFB1/4 Antibody
Catalog: DF3307
Description: Rabbit polyclonal antibody to PFKFB1/4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 54 KD; 55kD,54kD(Calculated).
Uniprot: P16118 | Q16877
RRID: AB_2835675

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PFKFB1/4 Antibody detects endogenous levels of total PFKFB1/4.
RRID:
AB_2835675
Cite Format: Affinity Biosciences Cat# DF3307, RRID:AB_2835675.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2; 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

Immunogens

Immunogen:
Uniprot:

P16118(F261_HUMAN) >>Visit HPA database.

Q16877(F264_HUMAN) >>Visit HPA database.

Gene(ID):
PFKFB1(5207) | PFKFB4(5210)
Expression:
P16118 F261_HUMAN:

Liver.

Sequence:
MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16118/Q16877 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S24 Phosphorylation
T39 Phosphorylation
T133 Phosphorylation
Y200 Phosphorylation
Y204 Phosphorylation
Y227 Phosphorylation
Y242 Phosphorylation
Y243 Phosphorylation
S262 Phosphorylation
K364 Ubiquitination
Y438 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
K13 Ubiquitination
K87 Ubiquitination
K101 Ubiquitination
K104 Ubiquitination
S119 Phosphorylation
T131 Phosphorylation
S176 Phosphorylation
S214 Phosphorylation
K265 Ubiquitination
S275 Phosphorylation
K283 Ubiquitination
K295 Ubiquitination
K305 Ubiquitination
K362 Ubiquitination

Research Backgrounds

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

Tissue Specificity:

Liver.

Subunit Structure:

Homodimer.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Function:

Synthesis and degradation of fructose 2,6-bisphosphate.

Subunit Structure:

Homodimer.

Family&Domains:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy. Molecular Medicine Reports, 2021 (PubMed: 34490476) [IF=3.4]

Application: WB    Species: Mouse    Sample: MPC5 cells

Figure 1. AR and PFKFB3 siRNAs are successfully transfected into MPC5 cells. MPC5 cells were treated with HG for 48 or 72 h. (A) Protein expression levels of enolase-1, PFKFB3, glyoxalase 1 and AR in MPC5 cells were detected by western blot analysis and normalized to GAPDH. (B) MPC5 cells were transfected with siRNA NC, siRNA AR or PFKFB3 siRNA1, −2 or −3 for 24 h. Then, the protein expression levels of PFKFB3 and AR were measured by western blot analysis and normalized to GAPDH. **P<0.01 vs. Control (NG). AR, aldose reductase; HG, high glucose; NC, negative control; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; siRNA, small interfering RNA.

Application: WB    Species: mouse    Sample: MPC5 cells

Figure 4.| Knockdown of PFKFB3 reverses podocyte injury through inhibition of the epithelial‑mesenchymal transition process. Protein expression levels of(A) PFKFB3, (B) WT‑1 and (C) E‑cadherin and α‑SMA in MPC5 cells were determined by western blot analysis and normalized to GAPDH.

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