NAB2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 cells using NAB2 antibody.

Product:	NAB2 Antibody
Catalog:	DF3298
Description:	Rabbit polyclonal antibody to NAB2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	55~70KD; 57kD(Calculated).
Uniprot:	Q15742
RRID:	AB_2835666

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

NAB2 Antibody detects endogenous levels of total NAB2.

RRID:

AB_2835666
Cite Format: Affinity Biosciences Cat# DF3298, RRID:AB_2835666.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EGR 1 binding protein 2; EGR-1-binding protein 2; EGR1 binding protein 2; MADER; Melanoma associated delayed early response protein; Melanoma-associated delayed early response protein; MGC75085; Nab 2; nab2; NAB2_HUMAN; NGFI A binding protein 2 (EGR1 binding protein 2); NGFI A binding protein 2; NGFI-A-binding protein 2; Protein MADER;

Immunogens

Immunogen:

A synthesized peptide derived from human NAB2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q15742(NAB2_HUMAN) >>Visit HPA database.

Gene(ID):

NAB2(4665)

Expression:

Q15742 NAB2_HUMAN:

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Sequence:

Q15742 NAB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHRAPSPTAEQPPGGGDSARRTLQPRLKPSARAMALPRTLGELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLFSQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVESVERIFRSFPRGDAGEVTSLLKLNKKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVARESTYLSSLKGSRLHPEELGGPPLKKLKQEVGEQSHPEIQQPPPGPESYVPPYRPSLEEDSASLSGESLDGHLQAVGSCPRLTPPPADLPLALPAHGLWSRHILQQTLMDEGLRLARLVSHDRVGRLSPCVPAKPPLAEFEEGLLDRCPAPGPHPALVEGRRSSVKVEAEASRQ

PTMs - Q15742 As Substrate

Q15742

Site	PTM Type	Source
S6	Phosphorylation	Uniprot
T8	Phosphorylation	Uniprot
S131	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
S162	Phosphorylation	Uniprot
K169	Sumoylation	Uniprot
K169	Ubiquitination	Uniprot
S171	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
S209	Phosphorylation	Uniprot
K269	Ubiquitination	Uniprot
S289	Phosphorylation	Uniprot
K361	Sumoylation	Uniprot
K361	Ubiquitination	Uniprot
K376	Acetylation	Uniprot
K376	Ubiquitination	Uniprot
K379	Sumoylation	Uniprot
T434	Phosphorylation	Uniprot
S471	Phosphorylation	Uniprot
S479	Phosphorylation	Uniprot
S514	Phosphorylation	Uniprot
S515	Phosphorylation	Uniprot
K517	Sumoylation	Uniprot

Research Backgrounds

Q15742_NAB2_HUMAN

Function:

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).

PTMs:

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.

Subcellular Location:

Nucleus.
Note: Isoform 2 is not localized to the nucleus.

Tissue Specificity:

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Subunit Structure:

Homomultimers may associate with EGR1 bound to DNA.

Family&Domains:

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.

The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Belongs to the NAB family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NAB2 Antibody - #DF3298